Induction of human endothelial cell apoptosis requires both heat shock and oxidative stress responses

The American Journal of Physiology
J H WangD Bouchier-Hayes

Abstract

Endothelial cell (EC) death may play an important role in the development of increased vascular permeability and capillary leak syndrome during systemic inflammatory response syndrome. However, the mode of EC death and the mechanisms involved remain unclear. In this study we employed the proinflammatory mediators lipopolysaccharide (LPS) and tumor necrosis factor-alpha (TNF-alpha), the chemical reagent sodium arsenite, and heat shock to trigger the stress gene responses. Human ECs were used as surrogates of the microvasculature to test the hypothesis that the induction of the heat shock response and the oxidative stress response might combine to induce apoptosis rather than necrosis in human ECs. Sodium arsenite at 80-320 microM, which induced heat shock protein 72 (HSP72) expression and reactive oxygen intermediate (ROI) generation in ECs, resulted in EC apoptosis. TNF-alpha alone (5-75 ng/ml) increased EC ROI generation but did not induce EC apoptosis. Heat shock alone (42 degrees C, 45 min) or sodium arsenite (40 microM) alone, each of which induced HSP72 expression, did not result in EC apoptosis. However, the combination of TNF-alpha with heat shock or 40 microM sodium arsenite led to EC apoptosis as HSP72 expression and R...Continue Reading

References

Jun 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M A Martin, H J Silverman
Nov 4, 1991·The American Journal of Cardiology·E G Nabel
Dec 1, 1990·Journal of Cellular Physiology·L S GibbsA B Malik
Apr 1, 1990·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·M A DeatonM C Powanda
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S M Keyse, R M Tyrrell
Jan 1, 1994·Journal of Neurochemistry·R R RatanJ M Baraban
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·C M Troy, M L Shelanski
May 15, 1993·Lancet·D A Carson, J M Ribeiro
Jul 1, 1993·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·B S PollaL Pizurki
Feb 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K HiroseK Matsushima
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L T ZhongD E Bredesen

❮ Previous
Next ❯

Citations

Jan 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S L DeMeesterJ P Cobb
May 13, 2006·Glycoconjugate Journal·Sergio F MartinSubroto Chatterjee
Dec 22, 1999·The American Journal of Physiology·Q D WuD Bouchier-Hayes
Jul 29, 2000·Leukemia·E M CreaghT G Cotter
Oct 8, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Colm P PowerH Paul Redmond
Sep 4, 2008·Antioxidants & Redox Signaling·Violetta N PivtoraikoJohn J Shacka
Oct 12, 2001·American Journal of Physiology. Endocrinology and Metabolism·A RissoA Ceriello
Oct 29, 2002·Bioscience, Biotechnology, and Biochemistry·Rumi IshisakaToshihiko Utsumi
Jun 13, 1998·The Journal of Biological Chemistry·J Cai, D P Jones
Sep 7, 2002·Antioxidants & Redox Signaling·Tadashi KasaharaYoshiko Sonoda
Dec 18, 2002·Annals of the New York Academy of Sciences·Hans W M NiessenC Erik Hack
Mar 27, 2004·Clinical Science·Ragheb A AssalyJ David Dignam
Jul 19, 2005·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Timothy A KingFrank G Ondrey
Nov 14, 1998·The American Journal of Physiology·J H WangD Bouchier-Hayes
Nov 30, 2000·Microbial Pathogenesis·E ValenteM C Plotkowski
May 16, 2002·Toxicology and Applied Pharmacology·Biing-Hui LiuYi-Ling Yang
Jun 22, 2002·Experimental Eye Research·Jennifer M RiegerJeffrey M Gidday
Jun 19, 2004·Free Radical Biology & Medicine·Ram KannanDavid R Hinton
Jan 18, 2006·Biochimica Et Biophysica Acta·Claudine TardyNathalie Andrieu-Abadie
Aug 11, 2006·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Min XiangZhiyu Qian

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis