Induction of human malignant T-lymphoblastic cell lines MOLT-3 and jurkat by 12-O-tetradecanoylphorbol-13-acetate: biochemical, physical, and morphological characterization

Journal of Cellular Physiology
K NagasawaT W Mak

Abstract

The process of induction of human malignant T-lymphoblastic cell line MOLT-3 by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) was examined. It was found that the induction process by TPA, which included increase in cells with receptors to sheep red blood cells (E--rosette positive--E+) and decrease in the levels of the marker enzyme terminal deoxynucleotidyl transferase (TdT) was not affected by the presence of DNA synthesis inhibitor arabinofuranosylcytosine (Ara-C). The exposure time to TPA required to elicit these changes was found to be short, in the order of 1 hour or less. The kinetics of the increased in E+ cells, decrease in the levels of TdT in these cells, or decrease in the ability to proliferate as measured by colony formation were similar with exposure to TPA for 1, 6, 24, or 96 hours. We have examined the effect of antitumor promoter compounds on their ability to block induction of MOLT-3 cells by TPA. Results indicated that none of these compounds, dexamethasone, antipain, retinoic acid, and L-1-tosylamide-2-phenylethylchloromethyl ketone (TPCK), was effective in reducing the number of E+ cells induced by TPA. Examination of three other leukemic T-cell lines indicated that, in addition to MOLT-3, t...Continue Reading

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S J CollinsR C Gallo
Feb 1, 1977·The Journal of Experimental Medicine·J L TouraineR A Good
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J Lotem, L Sachs
Jan 1, 1979·Clinical Immunology and Immunopathology·E W GelfandH M Dosch
Jun 14, 1979·The New England Journal of Medicine·G H ReamanD G Poplack
Sep 1, 1979·Journal of the National Cancer Institute·B E Chechik, J Minowada
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·N L EdwardsI H Fox
Jan 1, 1978·Annual Review of Biochemistry·P A Marks, R A Rifkind
Feb 1, 1979·International Journal of Cancer. Journal International Du Cancer·L C AnderssonC G Gahmberg
Aug 1, 1979·Experimental and Molecular Pathology·L F Skinnider
Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J LevyP A Marks
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J L WangG M Edelman
May 15, 1975·International Journal of Cancer. Journal International Du Cancer·J Lotem, L Sachs
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·P R McClintock, J Papaconstantinou
Sep 1, 1974·Experimental Cell Research·A M Mastro, G C Mueller
Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·C FriendT Sato
May 1, 1970·Medical & Biological Engineering·W B Taylor
Jan 1, 1980·Developmental Biology·R LevensonD Housman
Jun 16, 1980·Biochemical and Biophysical Research Communications·B P Alter, S C Goff
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·E J BenzR Hoffman
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P E Driedger, P M Blumberg
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K Nagasawa, T W Mak

❮ Previous
Next ❯

Citations

Jan 1, 1990·Journal of Clinical Laboratory Analysis·V M Marques-SilvaV M Rumjanek
Jun 1, 1992·Calcified Tissue International·T Ringbom-Anderson, K E Akerman
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B RyffelE Huberman
May 1, 1986·Journal of Cellular Physiology·L J Smith, E A McCulloch
Feb 1, 2019·Anti-cancer Drugs·Kok-Tong TanChi-Chien Lin
Jun 13, 1997·The Journal of Biological Chemistry·D M HaverstickL S Gray
May 1, 1992·Hematological Oncology·S KleinH Tesch
Feb 1, 1991·Molecular and Cellular Biology·C MurreD Baltimore

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved