Induction of human umbilical cord mesenchymal stem cells into tissue-forming cells in a murine model: implications for pelvic floor reconstruction

Cell and Tissue Research
Jing DingRobert Chun-Hua Zhao

Abstract

HUMSCs were isolated, differentiated and characterized in vitro. Both HUMSCs and smooth muscle cells differentiated from HUMSCs were used to fabricate tissue-engineered fascia equivalents. Forty-eight mature female Sprague Dawley rats were randomly assigned to four groups: group A (GynemeshTMPS, n = 12), group B (GynemeshTMPS + HUMSCs; n = 12), group C (GynemeshTMPS + smooth muscle cells differentiated from HUMSCs; n = 12) and group D (GynemeshTMPS + HUMSCs + smooth muscle cells differentiated from HUMSCs; n = 12). The posterior vaginal wall was incised from the introitus and the mesh was then implanted. Three implants of each type were tested at 1, 4, 8 and 12 weeks. Fibrotic remodeling, inflammation, vascularization and tissue regeneration were histologically assessed. The levels of type I and type III collagen were determined. There was no difference in fibrotic remodeling between cell-seeded and unseeded meshes at any time (p > 0.05). At 12 weeks, there did not appear to be fewer inflammatory cells around the filament bundles in the mesh with cells compared with the mesh alone (P > 0.05). Group D showed a trend toward better vascularization at 12 weeks compared with group A (P < 0.05). Twelve weeks after implantation, a thi...Continue Reading

References

Aug 1, 1993·The Journal of Surgical Research·D W FriedmanS B Deak
Feb 13, 1999·American Journal of Obstetrics and Gynecology·E C SamuelssonK F Svärdsudd
Sep 9, 2004·Current Opinion in Obstetrics & Gynecology·Narender N Bhatia, Mat H Ho
Sep 28, 2005·Best Practice & Research. Clinical Obstetrics & Gynaecology·Colin Birch
Nov 14, 2006·The Journal of Clinical Investigation·Jeffrey J RossCatherine M Verfaillie
Mar 31, 2007·Annals of the New York Academy of Sciences·Hans-Jörg BühringWichard Vogel
Feb 26, 2008·American Journal of Obstetrics and Gynecology·R Keith HuffakerLisa M Pierce
Mar 17, 2009·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Steven D AbramowitchPamela A Moalli
May 2, 2009·The Urologic Clinics of North America·Anthony Atala
May 4, 2010·Journal of Korean Medical Science·Joa-Jin LimKyung-Jin Lee
Oct 23, 2010·Obstetrics and Gynecology·Fiona J SmithNicolas Tsokos
Apr 21, 2011·International Urogynecology Journal·Hung-Jen WangMichael B Chancellor
May 13, 2011·The New England Journal of Medicine·Daniel AltmanUNKNOWN Nordic Transvaginal Mesh Group
Feb 26, 2010·World Journal of Stem Cells·Ching-Shwun Lin
Apr 4, 2012·Cell Transplantation·Hirotaka MasudaCaroline E Gargett
Aug 17, 2014·American Journal of Obstetrics and Gynecology·Rui LiangPamela A Moalli

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Citations

May 6, 2020·World Journal of Clinical Cases·Juan ChengYa-Li Miao
Sep 19, 2020·Cell & Bioscience·Yizhen Sima, Yisong Chen
Jul 28, 2021·Journal of Biomaterials Science. Polymer Edition·Deyu YangKehai Liu

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Methods Mentioned

BETA
flow cytometery
fluorescence microscopy
ELISA
electrophoresis

Software Mentioned

SPSS

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