Induction of humoral and cellular immunity against influenza virus by immunization of newborn mice with a plasmid bearing a hemagglutinin gene

International Immunology
A BotC Bona

Abstract

Neonates and infants display an intrinsic disability to mount protective immune responses to influenza viruses or conventional influenza vaccines. We investigated the ability of naked DNA to prime protective immune responses by inoculating newborn and adult mice with a plasmid (pHA) expressing hemagglutinin (HA) from the neurovirulent strain A/WSN/33 of influenza virus. Continuous exposure to small doses of antigen subsequent to neonatal DNA immunization led to effective priming of specific B and Th cells, rather than tolerance induction. The pHA immunization of adult mice primed a strongly biased Th1 response, whereas in neonates it induced a mixed Th1/Th2 response. In contrast to the effect of live-virus immunization, DNA immunization of neonates was followed by enhanced cytotoxic T lymphocyte responses subsequent to challenge with A/WSN/33 influenza virus. Mice immunized as neonates or adults with pHA plasmid exhibited significant increases in survival and decreases in virus lung titers following lethal challenge with the A/WSN/33 virus or the A/PR8/34 drift variant. Our results demonstrate that DNA vaccination is an efficient and safe means to generate broad humoral and cellular immune responses to influenza viruses, during...Continue Reading

Citations

Jun 24, 1999·Immunology Today·B Adkins
Sep 1, 2000·Advanced Drug Delivery Reviews·J Heppell, H L Davis
May 12, 2001·Vaccine·C A Siegrist
Aug 26, 1998·Vaccine·C ButtsM Sarzotti
Apr 13, 1999·Infectious Disease Clinics of North America·J R Haynes
Apr 5, 2002·Microbes and Infection·Adrian Bot, Constantin Bona
Dec 14, 1999·Microbes and Infection·H L Davis, M J McCluskie
Jan 25, 2005·The Pediatric Infectious Disease Journal·Stanley A Plotkin
Oct 22, 2005·Infection and Immunity·Adel M Talaat, Katherine Stemke-Hale
Dec 1, 2007·Genetic Vaccines and Therapy·Ana Cláudia PelizonAlexandrina Sartori
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·C L Brazolot MillanH L Davis
Apr 14, 2000·International Reviews of Immunology·S Fadel, M Sarzotti
Apr 14, 2000·International Reviews of Immunology·B Adkins
Apr 14, 2000·International Reviews of Immunology·A Bot
Apr 14, 2000·International Reviews of Immunology·J L Press
Jan 9, 2003·Expert Opinion on Biological Therapy·Adrian BotMatthias von Herrath
Jan 5, 2011·Immunological Reviews·Margaret A Liu
Jul 31, 2016·Homeopathy : the Journal of the Faculty of Homeopathy·Camila Monteiro SiqueiraCarla Holandino
Feb 5, 1999·European Journal of Immunology·B P ArulanandamD W Metzger
May 27, 2016·The Journal of Microbiology·Jae-Min Song
Sep 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shaza A FadelMarcella Sarzotti
Feb 17, 2000·The Journal of General Virology·A SolorzanoA García-Sastre
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·B P ArulanandamD W Metzger
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·B AdkinsP Guevara

❮ Previous
Next ❯

Related Concepts

Related Feeds