Induction of ID1 expression and apoptosis by the histone deacetylase inhibitor (trichostatin A) in human acute myeloid leukaemic cells.

Cell Proliferation
W-P YuW-F Dong

Abstract

ID1, founding member of the inhibitor of differentiation (ID) family, is involved in cell population growth, apoptosis and tumourigenesis. We investigated mRNA levels of ID1 in human myeloid leukaemic cell lines and in specimens of patients with acute myeloid leukaemia (AML), using semiquantitative reverse transcription-polymerase chain reaction, and protein levels of ID1 in human myeloid leukaemic cell lines using Western blot analysis. Six of seven AML cell lines and 12 of 15 AML patient samples were found to have barely detectable ID1 mRNA. All of these cell lines showed the same levels of protein in proportion to levels of mRNA. Two of the AML cell lines with low ID1 expression, KG1 and KG-1a, were chosen for treatment with either the DNA demethylation reagent, 5-aza-2'-deoxycytidine (DAC), or the histone deacetylase (HDAC) inhibitor, trichostatin A (TSA). These treatments were alone or in combination, and ID1 expression was induced by both DAC and TSA. No hypermethylated ID1 gene promoter was detected in the majority of the cell lines and patient specimens, by methylation-specific polymerase chain reaction, suggesting that induction of ID1 in KG1 and KG-1a was not due to direct demethylation of the ID1 gene promoter. Chrom...Continue Reading

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Citations

May 21, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Satoshi FujiiSubrata Sen
Jun 22, 2012·PloS One·Claudio D'AddarioMauro Maccarrone
Jun 12, 2002·Biochemical and Biophysical Research Communications·Xue-Qing WangSteven H Zuckerman
Nov 21, 2014·Journal of Clinical Biochemistry and Nutrition·Toshiko Suzuki-YamamotoYoshitaka Takahashi

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