PMID: 8939154Nov 1, 1996Paper

Induction of IgE antibodies in mice and rhesus monkeys with recombinant birch pollen allergens: different allergenicity of Bet v 1 and Bet v 2

The Journal of Allergy and Clinical Immunology
S VrtalaR Valenta

Abstract

Serologic measurements with recombinant birch pollen allergens, rBet v 1 and rBet v 2 (birch profilin), have shown that more than 95% of patients allergic to tree pollen mount high levels of IgE against rBet v 1, whereas only approximately 10% of the patients display rather low levels of IgE against rBet v 2. In this study an attempt was made to determine whether the different allergenicity of the major birch pollen allergen, rBet v 1, and a minor birch pollen allergen, rBet v 2, might be related to a different immunogenicity of the proteins as evaluated in experimental animal systems (mice and rhesus monkeys). Purified recombinant allergens were injected into mice and rhesus monkeys with aluminum hydroxide as adjuvant for elicitation of specific IgE responses. Antibody responses to the allergens were detected by immunoblotting, and time courses of immune responses were measured by ELISA. In both animal models more than the 10-fold dose of rBet v 2 was required to induce IgE antibodies, and even then, the amount of specific IgE antibodies elicited with rBet v 1 was substantially higher than that induced by rBet v 2. It was noted that rBet v 2 formed stable polymers through disulfide bonds. In two different animal models (mice a...Continue Reading

References

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Citations

Aug 5, 1998·Allergy·R ValentaD Kraft
Mar 3, 2010·Annual Review of Immunology·Rudolf ValentaSusanne Vrtala
Feb 16, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Nadine MothesSusanne Spitzauer
Dec 15, 2007·The Journal of Investigative Dermatology·Irene MittermannRudolf Valenta
Jan 22, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·W R Thomas
Aug 19, 2004·The Journal of Allergy and Clinical Immunology·Robert BünderHarald Renz
Oct 17, 2017·International Journal of Molecular Sciences·Wai Tuck SohHans Brandstetter
Jun 30, 2011·American Journal of Rhinology & Allergy·Martin CanisMatthias F Kramer
Aug 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Birgit LinhartRudolf Valenta
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Susanne VrtalaRudolf Valenta
Nov 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S VrtalaR Valenta
Mar 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Birgit LinhartThomas Wekerle

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