Induction of indoleamine 2,3-dioxygenase by gene delivery in allogeneic islets prolongs allograft survival

American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
H Dellê, I L Noronha

Abstract

Indoleamine 2,3-dioxygenase (IDO), an enzyme that plays a critical role in fetomaternal tolerance, exerts immunoregulatory functions suppressing T-cell responses. The aims of this study were to promote IDO expression in rat islets using a nonviral gene transfer approach, and to analyze the effect of the in vivo induction of IDO in a model of allogeneic islet transplantation. The IDO cDNA was isolated from rat placenta, subcloned into a plasmid and transfected into rat islets using Lipofectamine. The efficiency of transfection was confirmed by qRT-PCR and functional analysis. The in vivo effect of IDO expression was analyzed in streptozotocin-induced diabetic Lewis rats transplanted with allogeneic islets under the renal capsule. Transplantation of IDO-allogeneic islets reversed diabetes and maintained metabolic control, in contrast to transplantation of allogeneic nontransfected islets, which failed shortly after transplantation in all animals. Graft survival of allograft islets transfected with IDO transplanted without any immunosuppression was superior to that observed in diabetic rats receiving nontransfected islets. These data demonstrated that IDO expression induced in islets by lipofection improved metabolic control of st...Continue Reading

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Citations

Jan 13, 2012·Artificial Organs·Xiaoming DingJin Zheng
Nov 3, 2011·Journal of Transplantation·Preeti Chhabra, Kenneth L Brayman
Jun 13, 2012·International Journal of Endocrinology·Feng-Cheng ChouHuey-Kang Sytwu
Nov 5, 2011·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·F WanZ Dai
Jun 29, 2011·Arquivos De Gastroenterologia·Yngrid Ellyn Dias Maciel de SouzaLuiz Augusto Carneiro D'Albuquerque
Oct 25, 2011·Transplant International : Official Journal of the European Society for Organ Transplantation·Xiao-Hong WangJin Zheng
May 30, 2020·Artificial Cells, Nanomedicine, and Biotechnology·Hongchao PanAnquan Shang

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