Induction of inhibitory activity for B cell differentiation in human CD8 T cells with pokeweed mitogen, dimaprit, and cAMP upregulating agents: countersuppressive effect of platelet factor 4

Cellular Immunology
G M CrisiG J Thorbecke

Abstract

As shown previously, native or recombinant (r) human platelet factor 4 (PF4) alleviates the suppression induced by Con A or dimaprit, a histamine type 2 receptor (H2-R) agonist, in a murine system. The effect of rPF4 on human peripheral blood cells has now been studied, using as a model pokeweed mitogen (PWM)-induced, T-cell-mediated suppression of Ig-secreting cell (ISC) formation by Staphylococcus aureus and rIL-2 activated B cells. PWM, but not phytohemagglutinin (PHA), induced inhibitory activity in mitomycin-treated CD8+ T cells, but not unfractionated or CD4+ T cells, for both ISC formation and B cell proliferation. rPF4 and its C-terminal tridecapeptide alleviated the suppressive effect of PWM-activated CD8+ T cells on ISC production but not on proliferation. Heparin did not prevent this immunoregulatory activity of PF4. Neutralizing antibody to TGF-beta, but not to IFN-gamma or TNF-alpha, alleviated the suppression of ISC formation in some of the experiments. The H2-R appeared to play a part in inducing suppression, because the H2-R antagonist, cimetidine, prevented the PWM-induced suppression of ISC production. Furthermore, dimaprit induced suppression of ISC formation when added instead of PWM at the start of culture....Continue Reading

Citations

Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·L Z ChenG J Thorbecke
Sep 2, 1998·Mechanisms of Ageing and Development·G M CrisiG J Thorbecke
Jan 27, 1999·Arthritis and Rheumatism·G J ThorbeckeW J Simmons
May 19, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sandra C Silva-CardosoTimothy R D J Radstake
Oct 26, 2005·Journal of Leukocyte Biology·Mitsuyuki MuranoVincent K Tsiagbe
Sep 17, 2016·Journal of the American Heart Association·Yifang FanHeng Zhao
Dec 16, 2020·International Journal of Molecular Sciences·Antonino TuttolomondoAntonio Pinto

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