PMID: 2498451Jun 1, 1989Paper

Induction of interferon-gamma and tumor necrosis factor by Legionella pneumophila: augmentation of human neutrophil bactericidal activity

Journal of Leukocyte Biology
D K BlanchardJ Y Djeu

Abstract

We have previously reported that Legionella pneumophila antigens can induce interferon-gamma (IFN-gamma) and tumor necrosis factor (TNF) in vitro and in vivo in mice. Furthermore, treatment of murine polymorphonuclear leukocyte (PMN) cultures with these cytokines resulted in augmented killing of the bacteria in vitro. The purpose of the present study was to determine if these findings could be extended to human responses. Here we report that Legionella antigens induced IFN-gamma and TNF in nonimmune human leukocytes cultures, and that these cytokines were able to stimulate the bactericidal activity of isolated PMN against L. pneumophila in vitro. Furthermore, optimal production of IFN-gamma was found in cultures which were enriched for large granular lymphocytes (LGL). The phenotype of IFN-producing cells was determined to be CD11+, CD16+, CD2+, and negative for CD4, CD8, CD14, and Leu 7. Additionally, Legionella-infected monocytes were found to produce TNF in a dose-dependent response to the number of infecting bacteria, and the addition of recombinant IFN-gamma to infected monocytes resulted in augmented production of TNF in a synergistic manner. Finally, treatment of PMN with recombinant IFN-gamma and recombinant TNF augment...Continue Reading

Citations

Mar 9, 2011·Infection and Immunity·Kessler McCoy-SimandleNicholas P Cianciotto
Dec 1, 1992·The Journal of Pathology·M van DeurenJ W van der Meer
Jun 24, 2000·Proceedings of the Society for Experimental Biology and Medicine·S L McHughH Friedman
Feb 2, 2017·International Journal of Molecular Sciences·Manuela Di FrancoGiancarlo Iaiani
Feb 1, 1993·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·W HellR Marre
Apr 21, 2004·Immunology·Terri N Ellis, Blaine L Beaman
Oct 7, 2015·Frontiers in Immunology·Sylvia Simon, Hubert Hilbi
Jan 1, 1994·Biotherapy·T F Byrd
Feb 23, 2021·Frontiers in Cellular and Infection Microbiology·Tshegofatso NgwagaStephanie R Shames
Mar 1, 1992·Microbiological Reviews·J N DowlingR H Glew
Jun 6, 2003·Cytokine & Growth Factor Reviews·Klaus Pfeffer
Sep 2, 2021·Clinical & Translational Immunology·Lakshanie C WickramasingheMargaret L Hibbs

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