PMID: 9427519Jan 14, 1998Paper

Induction of interleukin-1 beta-converting enzyme (ICE) in murine microglia by lipopolysaccharide

Brain Research. Molecular Brain Research
J Yao, R W Johnson

Abstract

Interleukin-1beta (IL-1beta) synthesized in the brain is thought to be involved in the behavioral response to LPS. In this study, we examined in microglia the ability of LPS to induce interleukin-1 beta-converting enzyme (ICE), the protease responsible for processing proIL-1beta to its mature biologically active form. The murine microglial cell line, N13, and primary microglia which had been previously isolated from brains of 2-d-old endotoxin-responsive C3H/HeOuJ mice and endotoxin-resistant C3H/HeJ mice, were cultured in the presence of various concentrations of LPS. Oligonucleotide primers for ICE and GAPDH were used in RT-PCR to determine the levels of ICE mRNA in microglia. ICE mRNA was constitutively expressed in N13 cells and primary microglia from both C3H/HeOuJ and C3H/HeJ mice. Upon exposure to LPS, ICE mRNA levels were markedly elevated in N13 cells and in microglia from C3H/HeOuJ mice, but not in microglia from endotoxin-resistant C3H/HeJ mice. Western immunoblotting was used to determine if the increase in ICE mRNA induced by LPS was accompanied by an increase in ICE protein. A modest increase in immunoreactivity for the p45 ICE precursor protein was evident in N13 cells and primary microglia from C3H/HeOuJ mice fo...Continue Reading

References

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Citations

Dec 31, 2003·Journal of Neuroimmunology·Catharina LindbergMarianne Schultzberg
Jun 23, 1999·Journal of Neuroimmunology·S M Ye, R W Johnson
Jan 4, 2001·Trends in Neurosciences·N J Rothwell, G N Luheshi
May 18, 2000·Brain Research. Molecular Brain Research·J R HeyenR W Johnson
Apr 20, 2001·Brain Research. Molecular Brain Research·D C HarrisonK L Philpott
May 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Saebyeol JangRodney W Johnson
Apr 8, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Jing QinLin Lu
Jan 18, 2011·International Immunopharmacology·Na-Ra HanHyun-Ja Jeong
Jun 15, 2005·Journal of Neurochemistry·Kamil Pérez-CapoteCarme Solà
Sep 1, 2006·Journal of Neurochemistry·Anna K ClarkMarzia Malcangio
Jan 13, 1999·Clinical Immunology : the Official Journal of the Clinical Immunology Society·J D MarshallG Trinchieri
Aug 31, 2001·Microscopy Research and Technique·A K Cross, M N Woodroofe
Dec 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christian StockClaudia Eder
Apr 6, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·K FukuzukaD W Mozingo
Jan 15, 2020·Current Neuropharmacology·Mortimer Mamelak

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