Induction of interleukin-1 from murine peritoneal macrophages by Pseudomonas aeruginosa exotoxin A.

Infection and Immunity
M L MisfeldtR D LeGrand

Abstract

Pseudomonas exotoxin A, an ADP-ribosylating toxin produced by Pseudomonas aeruginosa, has been shown to stimulate the proliferation of murine thymocytes, which requires the participation of accessory cells. This requirement for accessory cells can be replaced by supernatant from adherent peritoneal exudate cells that have been stimulated with exotoxin A. Antibody to exotoxin A inhibits the induction of the thymocyte mitogenic activity from adherent peritoneal macrophages. However, antibody to exotoxin A had no effect on the thymocyte proliferation if the antibody was added to supernatant which contained thymocyte mitogenic activity. The thymocyte mitogenic activity was associated with a protein or protein complex with a molecular mass of greater than 10,000 daltons. D10 bioassays indicated the presence of interleukin-1 (IL-1) in the supernatant. Antibody to IL-1 inhibited the ability of supernatant to induce thymocytes to proliferate. Therefore, these data suggest that Pseudomonas exotoxin A can stimulate the production of IL-1 from adherent peritoneal cells, which induces murine thymocytes to proliferate.

References

Feb 1, 1989·Immunological Reviews·C A JanewayS Buxser
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P SchollT Chatila
Feb 1, 1988·Journal of Immunogenetics·C A JanewayS Buxser
Dec 1, 1988·European Journal of Immunology·A BauerH Kirchner
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Jul 1, 1984·Infection and Immunity·P S Holt, M L Misfeldt

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Citations

Jan 24, 2004·Clinical and Experimental Immunology·E J Giamarellos-BourboulisH Giamarellou
Jul 10, 1998·Immunology Letters·J MichalkiewiczK Madalinski
May 1, 1997·Toxicon : Official Journal of the International Society on Toxinology·A A Melo FilhoR A Ribeiro
Oct 1, 1991·Infection and Immunity·W A FlegelH Northoff
Aug 1, 1990·Infection and Immunity·M L Misfeldt

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