Induction of interleukin-8 expression in porcine peripheral blood mononuclear cells by trans10-cis12 conjugated linoleic acid

Veterinary Immunology and Immunopathology
Sung-Mok SonMhan-Pyo Yang

Abstract

The chemotaxis of porcine peripheral blood polymorphonuclear cells (PMN) was enhanced by culture supernatant from peripheral blood mononuclear cells (PBMC) treated with trans10-cis12 (10t-12c) conjugated linoleic acid (CLA) or by porcine recombinant (pr) interleukin (IL)-8. To identify this chemotactic inducing factor, the culture supernatant from 10t-12c CLA treated PBMC was partially purified by gel filtration. Only fraction 9 contained enhanced PMN chemotactic activity. The chemotactic activity of fraction 9 was inhibited by an anti-prIL-8 polyclonal antibody (pAb). Porcine IL-8 mRNA expression analyzed by a reverse transcriptase-polymerase chain reaction (RT-PCR) assay was increased in PBMC by the addition of 10t-12c CLA. This study strongly suggests that the immunoenhancing effect of CLA on the chemotactic response of porcine PMN is mediated through IL-8 produced by CLA treated PBMC.

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Citations

Jul 2, 2008·The British Journal of Nutrition·Uta EnkeUdo R Markert
Apr 2, 2013·The Journal of Nutritional Biochemistry·Monica ViladomiuJosep Bassaganya-Riera

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