Abstract
The aim of this study was to elucidate the role of IL-8 for neutrophil recruitment in nonallergic CRS patients. After coculture of Streptococcus pneumoniae (SP) with the mucosal epithelial cells (MECs) from non-CRS patients, at three different SP/MEC (1/1, 10/1, 100/1) ratios, the expression of IL-8 mRNA and the concentration of IL-8 were measured by RT-PCR and ELISA. The expression of CD11b/CD18 on neutrophils and E-selectin/ICAM-1 on endothelial cells and the adherence between neutrophils and human umbilical vascular endothelial cells (HUVECs) were determined by flow cytometric analysis, ELISA, and RIA, respectively. IL-8 concentration and IL-8 mRNA expression continued to increase from 3 hours after incubation in SP number-dependent manner. The expression of CD11b/CD18 on neutrophils and E-selectin/ICAM-1 on HUVECs, and the adherence between neutrophils and HUVECs were significantly increased in 10 SP/MEC-CM, and the increments were significantly blocked by anti-IL-8 antibody. MEC and IL-8 are major factors for neutrophil recruitment in nonallergic CRS.
References
Sep 1, 1996·The Journal of Allergy and Clinical Immunology·H SuzukiT Takasaka
Nov 27, 1998·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·A C Issekutz
Mar 9, 1999·The Journal of Allergy and Clinical Immunology·C RhyooD Proud
Aug 10, 2000·The Journal of Allergy and Clinical Immunology·D L Hamilos
Aug 23, 2001·Veterinary Parasitology·J P Dalton, G Mulcahy
Sep 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J A DiVietroM B Lawrence
Apr 10, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A M RiccioG Ciprandi
May 11, 2002·The Journal of Antimicrobial Chemotherapy·Tohru KikuchiToshihiro Nukiwa
Oct 9, 2002·The Laryngoscope·Ben WallworkAnders Cervin
Mar 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aihua LiRakesh K Singh
Oct 17, 2003·Current Drug Targets. Inflammation and Allergy·Hideaki Suzuki, Katsuhisa Ikeda
Sep 1, 2005·Angiogenesis·Aihua LiRakesh K Singh
Feb 10, 2006·The Laryngoscope·Ben WallworkAnders Cervin
Citations
Sep 21, 2011·Inflammation·Yaqing LiYingjie Xia
Mar 26, 2014·PloS One·Eliane KüngLucy J Hathaway
Apr 10, 2014·Current Allergy and Asthma Reports·Phil Lieberman, Debendra Pattanaik
Sep 13, 2016·International Forum of Allergy & Rhinology·Jenna M ChristensenRichard J Harvey
Sep 21, 2013·Molecular Medicine Reports·Jin ZhangXiang-Yang Li
Feb 6, 2020·Allergy, Asthma & Immunology Research·Zheng LiuLuo Zhang
Jul 30, 2020·International Forum of Allergy & Rhinology·Dong-Jin LimBradford A Woodworth
Jul 3, 2021·Medicina·Rudolfs Janis ViksneMara Pilmane