Induction of interleukin-8 in T84 cells by Vibrio cholerae.

Infection and Immunity
Xin ZhouJames B Kaper

Abstract

The induction of interleukin-8 (IL-8) in vitro has been suggested to correlate with the reactogenicity of Vibrio cholerae vaccine candidates. V. cholerae vaccine candidate 638, a hemagglutinin protease/hap-defective strain, was recently reported to be well tolerated in human volunteers, suggesting a role for Hap in reactogenicity. We examined the role of hap in the induction of IL-8 in intestinal epithelial T84 cells. Wild-type V. cholerae strains 3038 and C7258 and a vaccine candidate strain, JBK70, induced levels of IL-8 similar to those of their isogenic hap mutants. Supernatant containing Hap did not stimulate IL-8 production at a variety of concentrations tested, suggesting that Hap itself does not induce IL-8 production. Furthermore, supernatant from CVD115, which had deletions of hap and rtxA (encoding repeats in toxin) and was derived from a reactogenic strain, CVD110, induced IL-8 production in T84 cells in a dose-dependent manner. The IL-8-stimulating activity of CVD115 culture supernatants was growth phase dependent and was strongest in stationary phase cultures. This IL-8 stimulator(s) was resistant to heat treatment but sensitive to proteinase. Protease activity in vitro did not correlate with the reactogenicity of...Continue Reading

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Citations

Jun 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Karolis VaitkeviciusSun Nyunt Wai
Mar 10, 2016·Toxicon : Official Journal of the International Society on Toxinology·Jorge A Benitez, Anisia J Silva
Mar 4, 2008·Microbial Pathogenesis·Arunava BandyopadhayaKeya Chaudhuri
Sep 26, 2006·Molecular & Cellular Proteomics : MCP·Juan Xicohtencatl-CortésJorge A Girón
Sep 6, 2013·Microbiology and Molecular Biology Reviews : MMBR·Vanessa Liévin-Le Moal, Alain L Servin
Mar 19, 2014·FEMS Microbiology Letters·Daqing GaoChengping Lu

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