PMID: 9548558Apr 21, 1998Paper

Induction of interleukin (IL)-1 alpha and beta gene expression in human keratinocytes exposed to repetitive strain: their role in strain-induced keratinocyte proliferation and morphological change

Journal of Cellular Biochemistry
T TakeiB E Sumpio

Abstract

Recent studies in our laboratory have demonstrated that mechanical strain alters many facets of keratinocyte biology including proliferation, protein synthesis, and morphology. IL-1 is known to play an important role in the autocrine regulation of these basic cellular properties under basal and stimulated conditions. However, it is not known whether IL-1 plays a role in strain-induced alteration of keratinocyte biology. Thus, the objective of this study was to test the hypothesis that cyclic strain stimulates IL-1 expression and that strain-induced changes in keratinocyte function is regulated by IL-1. To test this hypothesis, we examined the effect of cyclic strain (10% average deformation) on keratinocyte IL-1 gene expression and the effect of neutralizing antibodies of IL-1 alpha and IL-1 beta on strain-induced changes in keratinocyte proliferation, morphology, and orientation. Northern blot analyses demonstrated that steady state levels of IL-1 alpha and beta mRNA were elevated by 4 h, peaked at 1 2 h of cyclic strain (IL-1 alpha, 304+/-14.2%; IL-1 beta, 212+/-5.6% increase vs. static controls) and decreased gradually by 24 h. IL-1 antibodies (IL-1 alpha, 0.01 microg/ml; IL-1 beta, 0.01 microg/ml) significantly blocked stra...Continue Reading

Citations

Dec 7, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jillian G RouseNancy A Monteiro-Riviere
Jun 20, 2007·Experimental Dermatology·Debbie BronnebergCatlijin V C Bouten
Mar 16, 2007·Experimental Dermatology·Mónika GöncziLászló Csernoch
Jan 22, 2003·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Frederick H SilverGurinder P Seehra
Jun 27, 1998·Biochemical and Biophysical Research Communications·M IkedaB E Sumpio
Feb 8, 2005·Environmental Management·Donald P AlthoffPhilip B Woodford

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