Induction of leptin expression in orbital preadipocyte fibroblasts

Thyroid : Official Journal of the American Thyroid Association
D Z EricksonRebecca S Bahn

Abstract

Graves' ophthalmopathy (GO) is an autoimmune disease characterized by an increase in the volume of the orbital fatty/connective tissues and extraocular muscles. This volume change is due to expansion of the adipose tissues and to accumulation of glycosaminoglycans and edema within the connective tissues of the orbit. We have shown previously that a subpopulation of confluent human orbital preadipocyte fibroblasts can be induced in vitro to differentiate into cells with morphological features of adipocytes and that these cultures express functional thyrotropin receptor (TSHR). In order to identify and study these cells further, we examined the expression of leptin protein and TSHR and leptin mRNA in these cultures. Using immunocytochemistry with objective measurement of immunofluorescent staining intensity on digitized microscopic images, we determined leptin protein expression to be 6 to 37 times greater in differentiated cultures than in control cultures. In addition, we showed that the expression of both genes is enhanced in differentiated cultures. We suggest that an unknown humoral stimulus, present in Graves' disease, might act to induce the differentiation of normal orbital fibroblasts into TSHR-bearing adipocytes. This p...Continue Reading

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Citations

Apr 16, 2002·Thyroid : Official Journal of the American Thyroid Association·Rebecca S Bahn
Dec 4, 2014·Metabolism: Clinical and Experimental·Claudio ProcacciniGiuseppe Matarese
Sep 10, 2004·Journal of Experimental Psychology. Learning, Memory, and Cognition·Lee-Xieng Yang, Stephan Lewandowsky
May 11, 2006·Endocrine Reviews·A SchäfflerC Büchler
May 15, 2021·Journal of Cellular and Molecular Medicine·Ziyi ChenYue Wang
Mar 30, 2021·European Thyroid Journal·Lisa Volke, Kerstin Krause

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