PMID: 2105292Feb 1, 1990Paper

Induction of levansucrase in Bacillus subtilis: an antitermination mechanism negatively controlled by the phosphotransferase system

Journal of Bacteriology
A M CrutzD Le Coq

Abstract

The target of the induction by sucrose of the levansucrase gene is a transcription terminator (sacRt) located upstream from the coding sequence, sacB. The two-gene locus sacX-sacY (formerly sacS) and the ptsI gene were previously shown to be involved in this induction. ptsI encodes enzyme I of the phosphoenolpyruvate-dependent phosphotransferase system. SacX is strongly homologous to sucrose-specific phosphotransferase system-dependent permeases. SacY is a positive regulator of sacB. Here we show that SacY is probably an antiterminator interacting directly with sacRt, since in Escherichia coli the presence of the sacY gene stimulates the expression of a reporter gene fused downstream from sacRt. Missense mutations affecting sacY were sequenced, and the sacB regulation was studied in isogenic strains carrying these mutations or in vitro-generated mutations affecting sacX, sacY, or ptsI. The phenotype of double mutants suggests a model in which SacX might be a sucrose sensor that would be phosphorylated by the phosphotransferase system and, in this state, could inhibit the SacY antiterminator. Exogenous sucrose, or a mutation inactivating the phosphotransferase system, would dephosphorylate SacX and allow antitermination at sacRt.

References

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Citations

Mar 16, 2017·Environmental Microbiology Reports·Jan Kampf, Jörg Stülke
Jan 1, 1996·Annual Review of Genetics·T M Henkin
Apr 20, 1995·Nature·A A AntsonP Gollnick
Oct 6, 2000·Annual Review of Microbiology·J Stülke, W Hillen
Jun 27, 2001·Journal of Biosciences·A Gulati, S Mahadevan
Apr 1, 1991·Molecular & General Genetics : MGG·K JahreisJ W Lengeler
Mar 10, 2005·The Journal of Biological Chemistry·Sharon Yagur-Kroll, Orna Amster-Choder
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·A Nussbaum-Shochat, O Amster-Choder
Jul 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hadas RavehOrna Amster-Choder
Feb 13, 2009·Journal of Applied Microbiology·M L Velázquez-HernándezJ J Valdez-Alarcón
Oct 28, 2009·Cellular and Molecular Life Sciences : CMLS·Pascale Romby, Emmanuelle Charpentier
Jan 2, 2015·Nucleic Acids Research·Da-Eun JeongSoo-Keun Choi
Jul 25, 2018·Journal of Bacteriology·Kambiz Morabbi Heravi, Josef Altenbuchner
Mar 8, 2002·Microbiology·María J GosalbesGaspar Pérez-Martínez
May 18, 2010·Journal of Basic Microbiology·María-Enriqueta Muñoz-Márquez, Elizabeth Ponce-Rivas

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