PMID: 20636264Jul 20, 2010Paper

Induction of lipid flip-flop by colicin E1 - a hallmark of proteolipidic pore formation in liposome membranes

Biochemistry. Biokhimii︠a︡
Alexander A SobkoYuri N Antonenko

Abstract

The addition of the channel-forming domain of colicin E1 to liposomes elicited the transmembrane diffusion (flip-flop) of lipids concomitant to the release of the fluorescent dye from liposomes. Good correlation was found between kinetic and concentration dependences of the two processes. Both the liposome leakage and the lipid flip-flop were stimulated upon alkalinization of the buffer solution after colicin binding at acidic pH. These results in combination with the analysis of the data on colicin binding to liposomes provide evidence in favor of the validity of the toroidal (proteolipidic) pore model as the mechanism of colicin channel formation.

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Citations

Nov 30, 2013·Biochimica Et Biophysica Acta·Yuri N AntonenkoMikhail P Kirpichnikov

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