Induction of luminol chemiluminescence by the manganese cluster of the photosystem II water-oxidizing complex to the S0, S1, S2, and S3 states

Biochemistry
I I Putrenko

Abstract

Luminol chemiluminescence (CL) (lambda(max) = 425 nm) induced by the manganese cluster of photosystem II (PSII) (t(max) = 1-5 min) along with CL induced by mono-, di-, or tetranuclear manganese coordination complexes (t(max) = 2-40 s) is observed when either 200 mM sodium phosphate or 1 M Tris-HCl + 200 microM EDTA are present in the reaction medium (pH 8.5) containing peroxidase. This light emission is not observed when Tris is used in the reaction medium without EDTA. The yield of a given CL without peroxidase is 5%-20% of that with peroxidase. The peroxidase-dependent CL is inhibited by catalase (I50 = 0.14-0.17 mu M), while the peroxidase-independent CL is not inhibited by 100 mM ethanol, 1 mM NaN3, 20 mu M Cyt c, or 0.6 mu M catalase. The CL induced by the Mn cluster of the water-oxidizing complex (WOC) in the S3 or S2 state exceeds that of lower S states by 15-20-fold. The magnitude of CL induced by Mn complexes is dependent on the ligand type of the complex. The ligand types of the Mn complexes and the WOC in different S states are ranked according to the magnitude of the induced CL: 1,10-phenanthroline > 2.2'-bipyridine > WOC (S3 or S2) > hydrotris(pirazolyl) borate > WOC (S0-2 or S0, S1) > 1,4,7-triazacyclononane. It i...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.