Induction of marked thrombin activity by pharmacologic concentrations of plasminogen activators in nonanticoagulated whole blood.

Thrombosis Research
P R Eisenberg, J P Miletich

Abstract

Thrombin activity reflected by increased plasma concentrations in vivo of fibrinopeptide A (FPA) increases when streptokinase (SK) is administered to patients with acute myocardial infarction. Although procoagulant effects have been found in vitro, the use of anticoagulated plasma limits the extent to which the phenomena observed can be viewed to implicate procoagulant effects in vivo. Accordingly, we characterized the procoagulant effects of SK and tissue plasminogen activator (t-PA) in nonanticoagulated whole blood. Blood was collected from normal volunteers by venipuncture (No. 19 gauge steel needle) directly into polypropylene tubes containing either t-PA, SK, SK and heparin, t-PA and heparin, or saline. The concentration of FPA after 10 min of incubation with saline was 150 +/- 46 nM (n = 14)(SE). In contrast, in blood incubated with SK FPA was consistently and markedly increased after 10 min: 2318 +/- 416 nM (100 IU/ml SK) and 10,889 +/- 1156 nM (1000 IU/ml SK) (p less than 0.001 compared with control). Less marked elevations of FPA occurred after 10 min in blood incubated with t-PA (3171 +/- 604 nM with 2500 ng/ml t-PA, p less than 0.01 compared with 1000 IU/ml SK). Increases in FPA were less than 100 nM in blood incubat...Continue Reading

References

Nov 1, 1988·The Journal of Clinical Investigation·J I WeitzT Eckhardt
May 1, 1985·Circulation·P R EisenbergA S Jaffe
Jul 1, 1974·The Journal of Clinical Investigation·H L NosselG D Qureshi
Sep 15, 1981·Analytical Biochemistry·J A Koehn, R E Canfield
Jan 1, 1963·Scandinavian Journal of Clinical and Laboratory Investigation·H C GODAL, I HELLE

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Citations

Jan 1, 1995·Journal of Thrombosis and Thrombolysis·H D White, S Yusuf
Apr 1, 1992·Journal of the American College of Cardiology·P R EisenbergA S Jaffe
Apr 1, 1992·Journal of the American College of Cardiology·S R TorrB E Sobel
Mar 31, 2004·American Journal of Cardiovascular Drugs : Drugs, Devices, and Other Interventions·John K FrenchJohn W Eikelboom
Sep 12, 2000·Current Cardiology Reports·J K French, H D White
Aug 1, 1991·The American Journal of Cardiology·B E Sobel, J Hirsh
Jul 1, 1995·Heart & Lung : the Journal of Critical Care·H BowlbyG D Clifton
Jan 25, 1991·The American Journal of Cardiology·P R Eisenberg
Dec 5, 2017·Anesthesiology·Jerrold H LevyNigel S Key
Nov 1, 1992·Circulation·D Waters, J Y Lam
May 20, 1999·American Journal of Hematology·H TsujiokaE Kakishita
Dec 16, 1993·The American Journal of Cardiology·P A MerliniP M Mannucci
Apr 1, 1992·Chest·P R Eisenberg

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