Induction of matrix metalloproteinase-1 by small interfering RNA targeting connective tissue growth factor in dermal fibroblasts from patients with systemic sclerosis

Experimental Dermatology
Hirohisa IshibuchiOsamu Ishikawa

Abstract

We aimed to evaluate the effect of small interfering RNA (siRNA) targeting CTGF on extracellular matrices (ECMs) metabolism in normal and SSc fibroblasts. Normal and SSc fibroblasts were transfected with CTGF-specific siRNAs to silence CTGF synthesis. After silencing CTGF, production of type I collagen and matrix metalloproteinase (MMP)-1 by fibroblasts stimulated with TGF-beta was examined. Then quantitative analyses of protein production or mRNA expression of type I collagen, MMP-1,-2,-9 and tissue inhibitor of metalloproteinase (TIMP)-1 with TGF-beta stimulation were carried out. Furthermore, after silencing CTGF, proliferations of normal and SSc fibroblasts were investigated. CTGF-specific siRNA significantly reduced CTGF production. The production of type I collagen was significantly reduced by CTGF silencing in normal fibroblasts. The CTGF silencing significantly increased the production of MMP-1 and decreased the production of TIMP-1 in SSc fibroblasts. The mRNA expression of MMP-1 was increased in CTGF-silenced SSc fibroblasts, but not in normal fibroblasts. There were no significant changes in the production or mRNA expression of other ECM-related genes in normal and SSc fibroblasts. Fibroblast proliferations were supp...Continue Reading

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Citations

Jun 14, 2013·PloS One·Hui-Min WangMei-Ling Ho
Sep 12, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Bruno Bordoni, Emiliano Zanier
Nov 6, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·Nandana BhardwajNam-Joon Cho
Jun 29, 2011·Experimental Dermatology·Pia MoinzadehNicolas Hunzelmann
Dec 1, 2012·Current Opinion in Nephrology and Hypertension·Susan V McLennanStephen M Twigg
May 10, 2020·BioMed Research International·Kornelia Kuźnik-TrochaKrystyna Olczyk

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