PMID: 9434138Jan 20, 1998Paper

Induction of megamitochondria by some chemicals inducing oxidative stress in primary cultured rat hepatocytes

Biochimica Et Biophysica Acta
M KarbowskiT Wakabayashi

Abstract

Effects of hydrazine, hydrogen peroxide and bromobenzene, inducers of free radicals, and those of erythromycin and cycloheximide, inhibitors of protein synthesis on structural changes of mitochondria in primary monolayer culture of rat hepatocytes were examined using laser confocal microscope and electron microscope. After 22 h of incubation of hepatocytes with 0.2 mM hydrogen peroxide or 10 microg ml-1 of erythromycin, mitochondria became extremely enlarged. Mitochondria of hepatocytes isolated from control rats became slightly to moderately enlarged in the presence of 2 mM hydrazine, while those of hepatocytes isolated from phenobarbital-pretreated animals became extremely enlarged in the presence of 2 mM hydrazine. Cycloheximide (0.5-10.0 microg ml-1) and bromobenzene (0.1-1.0 mM) failed to induce structural changes of mitochondria. The level of cytochrome P-450 in freshly prepared hepatocytes from phenobarbital-treated rats was 2.5 times higher than that from the control rats, and remained about three times higher than the latter after 22 h of incubation with 2 mM hydrazine. The level of malondialdehyde was invariably elevated when megamitochondria were induced. These results may suggest that oxidative stress is intimately ...Continue Reading

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Citations

Jan 26, 2000·Journal of Pediatric Surgery·C RomeoA Pierro
Jan 23, 1999·Free Radical Biology & Medicine·M KarbowskiT Wakabayashi
Oct 6, 2000·Biochimica Et Biophysica Acta·J P KamatT P Devasagayam
Mar 4, 2003·Journal of Cellular and Molecular Medicine·T Wakabayashi
Apr 26, 2008·Experimental Diabetes Research·Anders A F SimaHideki Kamiya
Dec 25, 2003·The Proceedings of the Nutrition Society·Simon Eaton
May 22, 2009·BioFactors·Nina A BonekampMichael Schrader
Sep 17, 2004·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·T G KlenøU G Sidelmann
Mar 16, 2000·Experimental and Molecular Pathology·M TeranishiT Wakabayashi
May 8, 2010·Toxicologic Pathology·Tomoya SanoIsao Narama
Dec 21, 2007·Antioxidants & Redox Signaling·Olga Martins de Brito, Luca Scorrano
May 28, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Kazushige OgawaFumihiko Sasaki
Mar 11, 2008·Rejuvenation Research·Carlo Bertoni-FreddariPatrizia Fattoretti
Apr 5, 2007·Annals of the New York Academy of Sciences·Carlo Bertoni-FreddariGemma Perretta
Nov 3, 2010·Transplant Infectious Disease : an Official Journal of the Transplantation Society·J-P LiuW-Z Ho
Sep 1, 2004·Journal of Hepatology·Victoria C CoggerDavid G Le Couteur

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