Induction of melanogenesis by rapamycin in human MNT-1 melanoma cells.

Annals of Dermatology
Young-Sool HahTae-Jin Yoon

Abstract

Melanogenesis is one of the characteristic parameters of differentiation in melanocytes and melanoma cells. Specific inhibitors of phosphatidylinositol 3-kinase (PI3K), such as wortmannin and LY294002, stimulate melanin production in mouse and in human melanoma cells, suggesting that PI3K and mammalian target of rapamycin (mTOR) might be involved in the regulation of melanogenesis. The involvement of the mTOR pathway in regulating melanogenesis was examined using human MNT-1 melanoma cells, and the effects of the potent inhibitor of mTOR, rapamycin, in the presence or absence of α-melanocyte-stimulating hormone (α-MSH) were evaluated. In cells treated with rapamycin, cell viability, melanin content, and tyrosinase (TYR) activity were measured and compared with untreated controls. Protein levels of TYR, tyrosinase-related protein (TYRP)-1, TYRP-2, and microphthalmia-associated transcription factor (MITF) were also analyzed by Western blot. In rapamycin-treated cells, the melanin content increased concomitantly with an elevation in TYR activity, which plays a major role in melanogenesis. There was also an up-regulation of TYR, TYRP-1, and MITF proteins. Combined treatment with rapamycin or wortmannin and α-MSH increased melanogen...Continue Reading

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Citations

Jan 31, 2014·Cellular and Molecular Life Sciences : CMLS·José A MartinaRosa Puertollano
Feb 13, 2013·The Journal of Cell Biology·Jose A Martina, Rosa Puertollano
May 27, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ji Yeon JeongMi Kyeong Lee
Feb 24, 2016·International Journal of Molecular Sciences·Magdalena DziałoAnna Kulma
Feb 27, 2016·Life Sciences·Eng-Soon KhorPooi-Fong Wong
Nov 8, 2018·Biological Chemistry·Sybille HasseThomas von Woedtke
Sep 24, 2016·Balsaeng'gwa saengsig·Sang Gu LimIn-Seok Park
Jan 21, 2020·Experimental Dermatology·Maja GrabackaKrzysztof Reiss
Aug 30, 2017·International Journal of Molecular Medicine·Nunzia CaporarelloCarmelina Daniela Anfuso
Aug 19, 2017·Experimental & Molecular Medicine·Kyu-Han KimEun-Gyung Cho
Mar 1, 2017·International Journal of Molecular Sciences·Jae Kyoung ChaeSun Yeou Kim
Mar 3, 2021·Journal of Pineal Research·Bernadetta BilskaKonrad Kleszczyński
Jul 13, 2021·Aging Cell·Josué Ballesteros-Álvarez, Julie K Andersen

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Methods Mentioned

BETA
protein assay
electrophoresis
dissecting

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