PMID: 9551360Apr 29, 1998Paper

Induction of melanoma antigen-specific cytotoxic T lymphocytes in vitro by stimulation with B7-expressing human melanoma cell lines

Journal of Immunotherapy
R G FentonD D Taub

Abstract

Crosslinking of CD28 receptors on resting T lymphocytes by B7 costimulatory molecules expressed by antigen-presenting cells (APCs) plays a critical role in T-cell activation. Human melanomas express major histocompatibility complex (MHC)-restricted tumor-associated antigens that can be recognized by cytotoxic T lymphocytes (CTL), yet they remain poorly immunogenic. One mechanism for the failure of T-cell response is the lack of expression of costimulatory molecules by human melanoma cells. We have transfected the B7-1 gene into three HLA-A2-expressing human melanoma cell lines, and studied their capacity to stimulate primary human T cells. B7-expressing melanoma cells were excellent inducers of T-cell proliferation, cytokine production, and cytolytic activity in allogeneic mixed lymphocyte cultures through a process dependent on the function of the T-cell receptor as well as interactions between B7:CD28, CD2:LFA-3, and LFA-1:ICAM-1. Subset analysis demonstrated that CD4+ T cells or addition of exogenous interleukin-2 was required for the induction of CD8+ CTL. Untransfected parental melanoma cells were inert as APCs in these cultures. Rotating stimulation of T cells with the three B7-expressing cell lines led to the generation ...Continue Reading

Citations

Feb 2, 2006·Cancer Immunology, Immunotherapy : CII·Lucas ChanFarzin Farzaneh
Feb 13, 2001·Molecular Immunology·A B AlexandroffD D Taub
Sep 28, 2000·Current Opinion in Immunology·R M Dallal, M T Lotze
Dec 25, 2007·American Journal of Reproductive Immunology : AJRI·Kunle Odunsi, Paul Sabbatini
Jul 10, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Paul Sabbatini, Kunle Odunsi
Jun 6, 2000·Journal of Immunotherapy·M S BradyJ S Lee

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