Induction of membrane circular dorsal ruffles requires co-signalling of integrin-ILK-complex and EGF receptor

Journal of Cell Science
S Babak AzimifarReinhard Fässler

Abstract

Integrin and receptor tyrosine kinase signalling networks cooperate to regulate various biological functions. The molecular details underlying the integration of both signalling networks remain largely uncharacterized. Here we identify a signalling module composed of a fibronectin-α5β1-integrin-integrin-linked-kinase (ILK) complex that, in concert with epidermal growth factor (EGF) cues, cooperatively controls the formation of transient actin-based circular dorsal ruffles (DRs) in fibroblasts. DR formation depends on the precise spatial activation of Src at focal adhesions by integrin and EGF receptor signals, in an ILK-dependent manner. In a SILAC-based phosphoproteomics screen we identified the tumour-suppressor Cyld as being required for DR formation induced by α5β1 integrin and EGF receptor co-signalling. Furthermore, EGF-induced Cyld tyrosine phosphorylation is controlled by integrin-ILK and Src as a prerequisite for DR formation. This study provides evidence for a novel function of integrin-ILK and EGF signalling crosstalk in mediating Cyld tyrosine phosphorylation and fast actin-based cytoskeletal rearrangements.

References

Jun 1, 1970·Experimental Cell Research·M AbercrombieS M Pegrum
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W S PearD Baltimore
Jun 30, 2000·Current Biology : CB·W T ArthurK Burridge
Oct 18, 2000·Proceedings of the National Academy of Sciences of the United States of America·A PfeiferI M Verma
Oct 30, 2001·The Journal of Biological Chemistry·June X ZouErkki Ruoslahti
May 17, 2002·Current Biology : CB·A Craig MackinnonBenjamin D Williams
Mar 13, 2003·Molecular Biology of the Cell·Eugene W KruegerMark A McNiven
Apr 3, 2003·EMBO Reports·Carsten GrashoffReinhard Fässler
May 26, 2004·Biochemical Society Transactions·S CabodiP Defilippi
Jun 2, 2004·Nature Reviews. Cancer·Timothy J Yeatman
Aug 4, 2004·The Journal of Cell Biology·Rebecca L BerdeauxG Steven Martin
Sep 16, 2004·Nature Reviews. Molecular Cell Biology·Roberto BuccioneMark A McNiven
Oct 22, 2004·The Journal of Biological Chemistry·William ReileyShao-Cong Sun
Mar 12, 2005·Cellular and Molecular Life Sciences : CMLS·S WiesnerR Fässler
May 4, 2005·The Journal of Cell Biology·Erik H J DanenArnoud Sonnenberg
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Kyle R LegateReinhard Fässler
Feb 24, 2006·Journal of Biomedical Science·Po-Chao ChanHong-Chen Chen
Jun 22, 2006·Journal of Cell Science·Laurence VeraciniChristine Benistant
Dec 2, 2006·Nature Reviews. Molecular Cell Biology·Matthias Mann
Dec 30, 2006·Molecular and Cellular Biology·Julio CastañoMireia Duñach
May 9, 2007·The Journal of Cell Biology·Katrin LorenzReinhard Fässler
Jul 28, 2007·Journal of the American Society of Nephrology : JASN·Yingjian LiYouhua Liu
Jan 17, 2008·Blood·Karissa D McCall-CulbreathMary M Zutter
Jan 29, 2008·The Journal of Biological Chemistry·Jinmin GaoJun Zhou
Feb 7, 2008·The Journal of Biological Chemistry·Yong-Bae KimJung Weon Lee
Mar 6, 2008·Journal of Cellular and Molecular Medicine·Michael Vibo Grandal, Inger Helene Madshus
Mar 12, 2008·The Journal of Cell Biology·Hao-Ven WangReinhard Fässler
Jun 11, 2008·The Journal of Cell Biology·Mark D BassMartin J Humphries
Dec 3, 2008·Cell Biochemistry and Biophysics·Payaningal R SomanathTatiana V Byzova
Jan 2, 2009·Journal of Cell Science·Kyle R Legate, Reinhard Fässler
Feb 26, 2009·Genes & Development·Kyle R LegateReinhard Fässler

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Citations

May 9, 2012·Nature Cell Biology·Ralph Thomas BöttcherReinhard Fässler
Nov 24, 2012·Journal of Biochemistry·Toshiki Itoh, Junya Hasegawa
Aug 29, 2012·Molecular and Cellular Biology·Jing-Ling HoonCheng-Gee Koh
Aug 20, 2014·Nature Communications·Thibaut EguetherAnne-Marie Tassin
Jun 15, 2013·Journal of Cell Communication and Signaling·Reyda Gonzalez-NievesMary L Cutler
Dec 19, 2013·Cellular and Molecular Bioengineering·John HuynhCynthia A Reinhart-King
Jul 15, 2015·Cellular Signalling·Ralph Christian Delos SantosCostin N Antonescu
May 20, 2016·Clinical & Translational Immunology·Jennifer L Stow, Nicholas D Condon
May 10, 2012·Cell Adhesion & Migration·Ernest Ho, Lina Dagnino
Oct 19, 2012·Cell Adhesion & Migration·Julia E SeroDonald E Ingber
Jul 6, 2012·Current Opinion in Cell Biology·Jun Qin, Chuanyue Wu
Aug 18, 2012·Current Opinion in Cell Biology·Maria Felice BrizziPaola Defilippi
Dec 14, 2016·Antioxidants & Redox Signaling·Gábor CsányiPatrick J Pagano
Sep 16, 2017·The Journal of Cell Biology·Ralph T BöttcherReinhard Fässler
Aug 11, 2016·F1000Research·Francois Bordeleau, Cynthia A Reinhart-King
Jan 30, 2019·The FEBS Journal·Ana Raquel RamosChristophe Erneux
May 20, 2017·Journal of the American Society of Nephrology : JASN·Archana RamanDarren P Wallace
Sep 21, 2019·The Journal of Cell Biology·Sarah KlapprothMarkus Moser
Aug 28, 2020·Journal of Cell Science·Anna V SamarelliRalph T Böttcher
Oct 4, 2019·Journal of Cell Science·Thomas BrombergerMarkus Moser
Apr 20, 2019·The European Journal of Neuroscience·Jun LiShigeo Okabe
Feb 12, 2017·Physical Review Letters·Erik Bernitt, Hans-Günther Döbereiner
Feb 1, 2014·Journal of Cell Science·James B ReineckeSteve Caplan
Jul 12, 2013·Journal of Cell Science·Daisuke HoshinoAlissa M Weaver
Jul 12, 2013·Journal of Cell Science·Nadav EladOhad Medalia
Jan 23, 2015·Journal of Cell Science·Esra KaraköseReinhard Fässler
Jul 11, 2021·Proceedings of the National Academy of Sciences of the United States of America·Daria Zdżalik-BieleckaMarta Miączyńska
Jan 9, 2022·Life Science Alliance·Ralph T BöttcherReinhard Fässler

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