PMID: 7526178Nov 1, 1994Paper

Induction of micronuclei by stilbene-type and steroidal estrogens in Syrian hamster embryo and ovine seminal vesicle cells in vitro

Mutation Research
R SchnitzlerM Metzler

Abstract

The induction of micronuclei (MN) is a known effect of the carcinogenic estrogen diethylstilbestrol (DES). We have now tested the time course and dose dependence of MN induction by DES and its analogs 3',3"-DES, indenestrol A (IA), indenestrol B (IB) or by the steroidal estrogen 17 beta-estradiol (E2) and by the clastogenic compound 4-nitroquinoline-N-oxide (NQO) in two primary mammalian cell culture systems. All compounds induced MN in Syrian hamster embryo and ovine seminal vesicle cells with compound-specific time courses and dose dependences. DES induced a maximum MN frequency 12 h post treatment, whereas with all other estrogens the highest MN frequency was observed 3-6 h after removal of the compound. The maximum MN frequency after NQO treatment occurred at 24 h or later. Of the stilbene estrogens tested, only DES caused an increase of the mitotic index. Further characterization of the MN by indirect immunofluorescence microscopy using CREST antikinetochore antibodies revealed that 92-99% of the DES-induced MN but only 0-2% of the NQO-induced MN contained CREST-reactive kinetochores. Since kinetochore-positive MN are indicative of whole chromosomes/chromatids and kinetochore-negative MN of acentric chromosomal fragments, ...Continue Reading

References

Jan 1, 1987·Cell Motility and the Cytoskeleton·W J WheelerB R Brinkley
Sep 1, 1986·Chemico-biological Interactions·J G LiehrA A Ulubelen
May 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J FothG H Degen

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Citations

Jun 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D Schiffmann, G H Degen
Apr 1, 1997·Environmental Health Perspectives·T Tsutsui, J C Barrett
Jun 5, 2004·Toxicology Letters·H F P JoostenE I Krajnc
Sep 18, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Hansi JiaYungang Liu

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