PMID: 7541752Jan 1, 1995Paper

Induction of micronuclei in murine lymphocytes by morphine

Environmental and Molecular Mutagenesis
S G Sawant, D B Couch

Abstract

Although individuals who abuse drugs are prone to an increased risk of malignancy, the mutagenic and carcinogenic potential of these agents has received relatively little attention. We report here on the potential of morphine to induce micronuclei in murine lymphocytes. Following a single intraperitoneal injection of 20 mg/kg morphine, the frequency of micronucleated binuclear (cytochalasin-blocked) murine T- and B-splenocytes was elevated from 12-36 hr after treatment. The maximum frequencies seen 24 hr after injection were 6.3- and 4.9-fold greater than the respective controls. A dose-dependent induction of micronuclei was observed from 5-20 mg/kg morphine, with no further increases in frequency produced by higher doses. In contrast, incubation of mitogen-stimulated splenocytes with 10(-7)-10(-4) M morphine in vitro produced no change in frequency of micronucleated cells relative to controls. Treatment with the narcotic antagonist naloxone (5 mg/kg) alone had no effect on the frequency of micronuclei, but reduced the clastogenic response of a subsequently administered dose of morphine (20 mg/kg). Thus, in murine lymphocytes morphine indirectly produces genetic damage, which is at least in part opioid receptor-mediated.

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Citations

Jan 1, 1996·Peptides·G A OlsonA J Kastin
Oct 24, 2001·Mutation Research·S G SawantD B Couch
Nov 1, 1996·Neurochemical Research·S Roy, H H Loh
Dec 28, 2007·Regulatory Toxicology and Pharmacology : RTP·Marilyn J AardemaGail Johnston
Nov 2, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Dana L ShueyRonald J Gerson
Dec 2, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Dana L ShueyRonald J Gerson
Oct 28, 2019·Naunyn-Schmiedeberg's Archives of Pharmacology·Thiago Kastell MazetoAlessandra Hubner de Souza
Oct 24, 2002·Journal of Acquired Immune Deficiency Syndromes : JAIDS·John J MaddenRobert M Donahoe
May 16, 2018·EFSA Journal·UNKNOWN EFSA Panel on Contaminants in the Food Chain (CONTAM)Christiane Vleminckx

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