Induction of MITF expression in human cholangiocarcinoma cells and hepatocellular carcinoma cells by cyclopamine, an inhibitor of the Hedgehog signaling

Biochemical and Biophysical Research Communications
Papavee SamatiwatS Shibahara

Abstract

Microphthalmia-associated transcription factor (MITF) is a key regulator of differentiation of melanocytes and retinal pigment epithelial cells, but it also has functions in non-pigment cells. MITF consists of multiple isoforms, including widely expressed MITF-A and MITF-H. In the present study, we explored the potential role played by the Hedgehog signaling on MITF expression in two common types of primary liver cancer, using human cholangiocarcinoma cell lines, the KKU-100 and HuCCT1, along with the HepG2 human hepatocellular carcinoma cell line. Importantly, cholangiocarcinoma is characterized by the activated Hedgehog signaling. Here we show that MITF-A mRNA is predominantly expressed in all three human liver cancer cell lines examined. Moreover, cyclopamine, an inhibitor of the Hedgehog signalling, increased the expression levels of MITF proteins in HuCCT1 and HepG2 cells, but not in KKU-100 cells, suggesting that MITF expression may be down-regulated in some liver cancer cases.

References

Sep 1, 1992·Journal of Gastroenterology and Hepatology·M R Haswell-ElkinsD B Elkins
Apr 5, 2000·The Journal of Biological Chemistry·K TakedaS Shibahara
Jan 5, 2002·The Journal of Investigative Dermatology. Symposium Proceedings·S ShibaharaK Takahashi
May 29, 2002·The EMBO Journal·Ken-ichi YasumotoShigeki Shibahara
Feb 1, 2003·Biochemical and Biophysical Research Communications·Kazuhisa TakedaShigeki Shibahara
Aug 29, 2003·Journal of Biochemistry·Hideo SaitoShigeki Shibahara
Oct 29, 2003·The Tohoku Journal of Experimental Medicine·Shigeki Shibahara
Jun 12, 2004·Seminars in Liver Disease·Yasser Shaib, Hashem B El-Serag
Sep 29, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Natini JinawathYusuke Nakamura
Mar 10, 2007·The Tohoku Journal of Experimental Medicine·Kazuhisa TakedaShigeki Shibahara
Apr 28, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Youngmi JungAnna Mae Diehl
Sep 17, 2008·Genes & Development·Markku Varjosalo, Jussi Taipale
Jan 21, 2010·Cancer Science·Hai-Rim ShinSung-Tae Hong
Jul 19, 2011·Biochimica Et Biophysica Acta·Yohei ShirakamiWilliam S Blaner
Jul 7, 2012·Pigment Cell & Melanoma Research·Julien MaruottiNoriko Esumi
Nov 3, 2012·The Tohoku Journal of Experimental Medicine·Soisungwan Satarug, Michael R Moore
Mar 16, 2013·Occupational and Environmental Medicine·Shinji KumagaiGaku Ichihara
Jan 15, 2014·Journal of Hepato-biliary-pancreatic Sciences·Shoji KuboGinji Endo
Apr 16, 2014·Journal of Hepato-biliary-pancreatic Sciences·Dorothee RiedlingerCarsten Kamphues
Apr 24, 2014·Journal of Agricultural and Food Chemistry·Stephen T LeeCheng-Wei Tom Chang
Dec 3, 2014·Biochemical and Biophysical Research Communications·Wipa ChantarawongShigeki Shibahara
Feb 25, 2015·Naunyn-Schmiedeberg's Archives of Pharmacology·Papavee SamatiwatVeerapol Kukongviriyapan
Nov 3, 2015·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Koji OhbaShigeki Shibahara

❮ Previous
Next ❯

Citations

Jul 8, 2016·Experimental Biology and Medicine·Katrin ZeilingerFanny Knöspel
Nov 20, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Koji OhbaShigeki Shibahara

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.