Induction of monocyte tissue factor expression by antibodies to heparin-platelet factor 4 complexes developed in heparin-induced thrombocytopenia.

Blood
C PouplardY Gruel

Abstract

The pathogenesis of thrombosis in heparin-induced thrombocytopenia (HIT) was studied by investigating whether antibodies to heparin-platelet factor 4 (H-PF4) induced tissue factor (TF) synthesis by monocytes. Plasma from 5 patients with HIT containing IgG to H-PF4 was incubated with peripheral blood mononuclear cells without or with purified PF4 and heparin. Significant TF-dependent procoagulant activity (PCA) expressed by monocytes, measured with a factor Xa-based chromogenic assay, was induced after incubation of each HIT plasma sample. This monocyte PCA required the presence of PF4 and was inhibited by high concentrations of heparin. Furthermore, purified HIT IgG added to whole blood with PF4 and heparin also provoked significant synthesis of TF mRNA by monocytes, demonstrated by RT-PCR, and this effect was not observed with normal IgG. These findings strongly support the hypothesis that antibodies to PF4 developed in HIT trigger the production of tissue factor by monocytes, and this effect could account in vivo for hypercoagulability and thrombotic complications in affected patients.

References

Mar 5, 1987·The New England Journal of Medicine·D B CinesS Tannenbaum
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·A CeliB Furie
Jun 1, 1995·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·B Osterud
Jan 1, 1996·Thrombosis Research·E CamererH Prydz

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Citations

Jun 13, 2006·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Emmanuel de MaistreDominique Lasne
Feb 18, 2011·Journal of Thrombosis and Thrombolysis·Adam Cuker
May 20, 2006·Thrombosis Research·Lorenz RischMarkus Schmugge
Jan 22, 2003·Seminars in Dialysis·Robert F Reilly
Aug 13, 2002·Current Opinion in Hematology·Michael P Reilly, Steven E McKenzie
Aug 13, 2002·Current Opinion in Pulmonary Medicine·Theodore E Warkentin
Aug 12, 2005·Current Opinion in Pulmonary Medicine·Jeanine M WalengaMamdouh Bakhos
Aug 2, 2007·Journal of Infusion Nursing : the Official Publication of the Infusion Nurses Society·Joseph M Swanson
Feb 5, 2005·Pathophysiology of Haemostasis and Thrombosis·Norbert Lubenow
Sep 24, 2008·Blood·John G Kelton, Theodore E Warkentin
Jun 2, 2012·Blood·Steven E McKenzie
Mar 28, 2002·Clinical Chemistry and Laboratory Medicine : CCLM·Anna YuNils Egberg
Jul 6, 2014·Current Opinion in Hematology·Steven E McKenzie, Bruce S Sachais
May 4, 2013·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Yuval RamotGalia Spectre
Aug 10, 2010·Der Internist·T ThieleA Greinacher
Sep 22, 2005·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Meganathan KannanRenu Saxena
Sep 12, 2002·Expert Opinion on Therapeutic Targets·Donald S Houston
Apr 28, 2005·Expert Review of Cardiovascular Therapy·Peter A Kouides, William Fricke
Jul 1, 2004·Expert Review of Cardiovascular Therapy·Beng H Chong, James J H Chong
Mar 25, 2009·Expert Opinion on Drug Safety·Theodore E Warkentin, Andreas Greinacher
Jun 20, 2003·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Harry MessmoreJeanine Walenga
Oct 1, 2013·La Revue de médecine interne·Y GruelC Pouplard
Sep 24, 2011·International Journal of Inflammation·Arthur J Chu
Jun 15, 2012·Thrombosis Research·Per Morten Sandset
Dec 17, 2009·Thrombosis Research·M Anna KowalskaMortimer Poncz
Mar 9, 2010·European Journal of Internal Medicine·Jecko Thachil
Nov 1, 2006·Critical Care Medicine·Lena M NapolitanoStanley A Nasraway
Jan 22, 2008·Hematology/oncology Clinics of North America·Debra A Hoppensteadt, Jeanine M Walenga
Aug 18, 2005·Seminars in Thoracic and Cardiovascular Surgery·Mortimer Poncz
Jun 30, 2004·American Journal of Hematology·Lucie Opatrny, Margaret N Warner
Oct 26, 2005·American Journal of Hematology·Martine Szyper Kravitz, Yehuda Shoenfeld
Jul 28, 2009·Journal of Thrombosis and Haemostasis : JTH·L RauovaM Poncz
May 20, 2003·British Journal of Haematology·Theodore E Warkentin

❮ Previous
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