Induction of mRNA expression of osteogenesis-related genes by guaiacol in human dental pulp cells.

Odontology
Takashi KatoTakeki Tsutsui

Abstract

To investigate the stimulating effect of endodontic medications on the mRNA expression of some osteogenesis-related genes associated with reparative dentinogenesis and hard-tissue formation, human dental pulp cells (D824 cells) were treated with calcium hydroxide (Ca (OH)(2)), formocresol, or guaiacol. The effect on growth was determined by growth curves of D824 cells treated for 1-3 days with 0.03-0.3 mM Ca (OH)(2), 0.0007%-0.0014% formocresol, or 0.24-2.43 mM guaiacol. The mitotic activity of individual cells and the mRNA expression of the osteogenesis-related genes for alkaline phosphatase (ALP), type I collagen (COL-1), and bone sialoprotein (BSP) in the cells treated for 24 h with the same concentrations of the medications as described above were determined by colony-forming efficiency and by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis, respectively. Cellular growth and mitotic activity were scarcely affected by Ca (OH)(2), but were significantly reduced by formocresol or guaiacol. The mRNA expression of the osteogenesis-related genes was little affected by Ca (OH)(2) or formocresol, but was significantly enhanced by guaiacol. The results indicate that guaiacol may stimulate th...Continue Reading

References

Jul 1, 1982·Oral Surgery, Oral Medicine, and Oral Pathology·K W CunninghamD M Ranly
Feb 24, 2001·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·J E Aubin, E Bonnelye
Feb 24, 2001·Journal of Endodontics·Y C ChangM F Huang
Nov 3, 2006·Biochemical Pharmacology·Nobuaki MoriguchiYukio Yoneda
Sep 18, 2008·Indian Journal of Dental Research : Official Publication of Indian Society for Dental Research·Zahra BahrololoomiGhofran Hosseini
Nov 26, 2008·Journal of Endodontics·Raquel Assed Bezerra da SilvaPaulo Tambasco de Oliveira

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Citations

Oct 22, 2013·Toxicology and Applied Pharmacology· SurigugaZong-Chun Yi
Feb 10, 2019·Oral and Maxillofacial Surgery·Patricia Verónica Aulestia-VieraMaria Aparecida Borsatti

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