Induction of multifunctional human immunodeficiency virus type 1 (HIV-1)-specific T cells capable of proliferation in healthy subjects by using a prime-boost regimen of DNA- and modified vaccinia virus Ankara-vectored vaccines expressing HIV-1 Gag coupled to CD8+ T-cell epitopes.

Journal of Virology
N GoonetillekeAndrew J McMichael

Abstract

A double-blind randomized phase I trial was conducted in human immunodeficiency virus type 1 (HIV-1)-negative subjects receiving vaccines vectored by plasmid DNA and modified vaccinia virus Ankara (MVA) expressing HIV-1 p24/p17 gag linked to a string of CD8(+) T-cell epitopes. The trial had two groups. One group received either two doses of MVA.HIVA (2x MVA.HIVA) (n=8) or two doses of placebo (2x placebo) (n=4). The second group received 2x pTHr.HIVA followed by one dose of MVA.HIVA (n=8) or 3x placebo (n=4). In the pTHr.HIVA-MVA.HIVA group, HIV-1-specific T-cell responses peaked 1 week after MVA.HIVA vaccination in both ex vivo gamma interferon (IFN-gamma) ELISPOT (group mean, 210 spot-forming cells/10(6) cells) and proliferation (group mean stimulation index, 37), with assays detecting positive responses in four out of eight and five out of eight subjects, respectively. No HIV-1-specific T-cell responses were detected in either assay in the 2x MVA.HIVA group or subjects receiving placebo. Using a highly sensitive and reproducible cultured IFN-gamma ELISPOT assay, positive responses mainly mediated by CD4(+) T cells were detected in eight out of eight vaccinees in the pTHr.HIVA-MVA.HIVA group and four out of eight vaccinees in...Continue Reading

References

Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D RichmanJ Griffin
Jun 23, 1994·Nature·L L LauR Ahmed
Jan 1, 1995·Nature Medicine·S Rowland-JonesT Corrah
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·J L HeeneyD Davis
Mar 12, 1999·Science·M CarringtonS J O'Brien
Nov 13, 1999·Science·K Murali-KrishnaR Ahmed
Mar 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·S A MiguelesM Connors
Jul 6, 2000·The Journal of Experimental Medicine·V AppayS L Rowland-Jones
Nov 22, 2000·The Journal of Clinical Investigation·M F CallanA J McMichael
Mar 10, 2001·Nature·P ChampagneG Pantaleo
Apr 3, 2001·Science·D Paydarfar, W J Schwartz
Oct 20, 2001·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·R M RuprechtH M McClure
Jan 17, 2002·Journal of Immunological Methods·Jeffrey R CurrierJosephine H Cox
Feb 28, 2002·Annual Review of Immunology·Norman L LetvinDavid C Montefiori
May 2, 2002·Vaccine·Tomás HankeRachel V Samuel
Jun 25, 2002·AIDS Research and Human Retroviruses·Matilu MwauTomás Hanke
Jul 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Luwy MuseyM Juliana McElrath

❮ Previous
Next ❯

Citations

Jan 16, 2007·Cancer Immunology, Immunotherapy : CII·Guang-Yun SongJoshua D I Ellenhorn
Mar 21, 1998·Cellular Signalling·M G Thompson, R M Palmer
Aug 4, 2010·Immunology and Cell Biology·Paweena RattanasenaAndreas Suhrbier
Feb 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Max CorbettAlain Le Pape
Jun 3, 2009·The Journal of Experimental Medicine·Nilu GoonetillekeAndrew J McMichael
May 1, 2008·The Journal of Infectious Diseases·Mary K WlochMichael Boeckh
Sep 24, 2008·The Journal of Infectious Diseases·Eric SandströmUNKNOWN HIV Immunogenicity Study 01/02 Team
Jun 14, 2013·AIDS·Laura RichertUNKNOWN ANRS Vaccine Network/Vaccine Research Institute
May 15, 2009·Journal of Virology·Bette T KorberBarton F Haynes
Nov 23, 2007·Journal of Virology·Suzanne L ElliottMandvi Bharadwaj
Jun 6, 2007·Doklady. Biochemistry and Biophysics·L I KarpenkoL S Sandakhchiev
Jan 2, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Cornelia L TrimbleT C Wu
Dec 25, 2010·The Journal of Clinical Investigation·Stuart N Isaacs
Dec 12, 2012·The Journal of Clinical Investigation·Michael K P LiuNilu Goonetilleke
Jul 23, 2013·Retrovirology·Torben SchiffnerLucy Dorrell
May 9, 2008·PLoS Computational Biology·David C NickleJames I Mullins
Oct 4, 2007·PloS One·Sven LétourneauTomás Hanke

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