Induction of NADPH oxidase activity and reactive oxygen species production by a single Trypanosoma cruzi antigen

International Journal for Parasitology
Natalia GuiñazúSusana Gea

Abstract

Trypanosoma cruzi is an intracellular protozoan parasite that predominantly invades mononuclear phagocytes and is able to establish a persistent infection. The production of reactive oxygen species (ROS) by phagocytes is an innate defence mechanism against microorganisms. It has been postulated that ROS such as superoxide anion (O(2)), hydrogen peroxide and peroxynitrite, may play a crucial role in the control of pathogen growth. However, information on parasite molecules able to trigger ROS production is scarce. In this work, we investigated whether cruzipain, an immunogenic glycoprotein from T. cruzi, was able to trigger the oxidative burst by murine cells. By employing chemiluminiscense and flow-cytometric analysis, we demonstrated that cruzipain induced ROS production in splenocytes from non-immune and cruzipain immune C57BL/6 mice and in a Raw 264.7 macrophage cell line. We also identified an O(2)(-) molecule as one of the ROS produced after antigen stimulation. Cruzipain stimulation induced NOX2 (gp91(phox)) and p47(phox) expression, as well as the co-localisation of both NADPH oxidase enzyme subunits. In the current study, we provide evidence that cruzipain not only increased ROS production but also promoted IL-6 and IL-...Continue Reading

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Citations

Dec 27, 2011·Molecular & Cellular Proteomics : MCP·Jian-Jun Wen, Nisha Jain Garg
Sep 3, 2013·Antioxidants & Redox Signaling·Claudia N Paiva, Marcelo T Bozza
Apr 3, 2016·Mutation Research. Reviews in Mutation Research·Alice Machado-SilvaCarlos Renato Machado
Oct 26, 2013·Journal of Receptor and Signal Transduction Research·Song Mao, Songming Huang
Apr 20, 2018·PLoS Pathogens·Claudia N PaivaMarcelo T Bozza
Apr 21, 2020·Oxidative Medicine and Cellular Longevity·Juana P Sánchez-VillamilNisha J Garg
Nov 17, 2020·Frontiers in Microbiology·Javier UrquizaJorge Quarleri

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