Induction of nitric oxide and respiratory burst response in activated goldfish macrophages requires potassium channel activity

Developmental and Comparative Immunology
James L StaffordMiodrag Belosevic

Abstract

Potassium channel activity is important for modulating mammalian macrophage antimicrobial functions. The involvement of potassium channels in mediation of immune cell function in lower vertebrates, such as teleost, has not been explored. Since relatively little is known about the types of potassium channels present in fish macrophages, pharmacological blockers with broad ranges of activity were tested: 4-aminopyridine (4-AP), quinine, and tetraethylammonium chloride (TEA). The potassium channel blockers inhibited reactive nitrogen intermediates (RNI) and reactive oxygen intermediates (ROI) production by goldfish macrophages activated with bacterial lipopolysaccharide (LPS) and/or macrophage activating factor (MAF)-containing supernatants. Quinine was the most potent inhibitor with an IC(50) of 50 microM, while the other blockers, 4-AP and TEA, had IC(50) of 1.2 and 0.6mM, respectively. A reversible depolarization of the goldfish macrophage plasma membrane potential (Vm) was observed following treatments with potassium channel blockers, and was related to transcriptional changes in the inducible nitric oxide synthase gene (iNOS). Down-regulation of antimicrobial activities and depolarization of the goldfish macrophage plasma mem...Continue Reading

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Citations

Jun 12, 2003·Developmental and Comparative Immunology·James L StaffordBrad G Magor
Dec 4, 2015·Biology·Jordan W HodgkinsonMiodrag Belosevic
Mar 19, 2011·Developmental and Comparative Immunology·Maria ForlenzaGeert F Wiegertjes
Mar 19, 2011·Developmental and Comparative Immunology·Aja M Rieger, Daniel R Barreda
Feb 3, 2005·Fish & Shellfish Immunology·Patrick C Hanington, Miodrag Belosevic
Jun 28, 2005·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·F B Eddy
Nov 6, 2008·Fish & Shellfish Immunology·Patrick C HaningtonMiodrag Belosevic
Jun 13, 2018·Frontiers in Immunology·Leon GrayferMiodrag Belosevic
Apr 11, 2021·Fish & Shellfish Immunology·Silas F EtoJoão M Pizauro

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