Induction of nitric oxide synthesis in murine macrophages by Helicobacter pylori

Cancer Letters
K B Shapiro, J H Hotchkiss

Abstract

Intact Helicobacter pylori cells, as well as cellular components, stimulated nitric oxide (NO) synthesis in an in vitro murine macrophage system by the L-arginine-nitric oxide pathway. Macrophage-mediated NO formation was dependent on the presence of H. pylori and exhibited a dose-dependent increase at H. pylori concentrations between 10(6) and 5 and 10(7) cells/ml. H. pylori mediated NO synthesis also required L-arginine and was inhibited by NG-monomethyl-L-arginine (NMMA), a selective inhibitor of nitric oxide synthase. NO synthesis was induced by whole H. pylori cells. H. pylori media filtrate, extracted membrane proteins, and H. pylori lipopolysaccharide (LPS). Maximal NO synthesis was induced by viable H. pylori cells with media filtrate and membrane protein extracts inducing significant NO responses. NO stimulation by media filtrate and membrane protein extracts support secreted H. pylori products as potential activators of inflammatory cell NO synthesis in vivo. NO synthesis in response to H. pylori suggests that chronic H. pylori infection may increase endogenous formation of NO. Elevated NO exposure may represent an etiologic factor explaining the epidemiologic association between long-term H. pylori infection and gast...Continue Reading

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·T NguyenS R Tannenbaum
Nov 1, 1991·Annals of Surgery·J B OchoaA B Peitzman
Oct 15, 1990·International Journal of Cancer. Journal International Du Cancer·D FormanJ Chen
Jul 1, 1987·Carcinogenesis·M MiwaS R Tannenbaum
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R IyengarM A Marletta
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Jun 1, 1995·Mutation Research·R H Liu, J H Hotchkiss
Jul 1, 1994·The Journal of Infectious Diseases·H NielsenA P Moran
Jan 1, 1995·Mediators of Inflammation·P G JorensH Bult
Aug 9, 2013·The American Journal of Tropical Medicine and Hygiene·Khitam Muhsen, Dani Cohen

❮ Previous
Next ❯

Citations

Oct 21, 1999·Cancer Letters·C DoiT Ito
Nov 5, 1997·Journal of Pain and Symptom Management·M Anbar, B M Gratt
Apr 23, 2002·Alimentary Pharmacology & Therapeutics·A ShiotaniM Ichinose
Nov 28, 2002·International Journal of Dermatology·Mehmet Ali GürerCiğdem Atahan
Mar 29, 2000·Journal of Gastroenterology and Hepatology·S WatanabeT Miwa
Apr 12, 2000·Journal of Gastroenterology and Hepatology·H Suzuki, H Ishii
Nov 3, 2010·Scandinavian Journal of Immunology·T Qidwai, F Jamal
Jun 12, 2004·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·A ShiotaniY Nakae
Jan 13, 2015·Gastroenterology Research and Practice·Seyyed Ali MardBijan Ahmadi
Sep 6, 2000·Scandinavian Journal of Gastroenterology·M IguchiS Nishioka
Jun 11, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alain P GobertKeith T Wilson
Jan 31, 2002·World Journal of Gastroenterology : WJG·Zun-Wu Zhang, Michael JG Farthing

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.