Induction of obesity impairs reverse cholesterol transport in ob/ob mice

PloS One
MyNgan DuongStephen J Nicholls

Abstract

Obesity is an independent risk factor for cardiovascular disease. Reverse cholesterol transport (RCT) is an important cardioprotective mechanism. This study aimed to investigate RCT changes in a murine model of obesity. Ob/ob and control mice were injected with [3H]-cholesterol-labelled macrophages and cholesterol accumulation quantified after 48 h. Ex vivo, cholesterol efflux and uptake were determined in hepatic and adipose tissues. Ob/ob mice had more labelled cholesterol in their plasma (86%, p<0.001), suggesting impaired RCT. SR-BI-mediated cholesterol efflux was elevated from ob/ob mice (serum, 33%; apoB-depleted plasma, 14%, p<0.01) and HDL-c were also higher (60%, p<0.01). Ex vivo it was found that cholesterol uptake was significantly lower into the livers and adipose tissue of ob/ob mice, compared to non-obese wildtype controls. Furthermore, ex vivo cholesterol efflux was reduced in ob/ob liver and adipose tissue towards apoA-I and HDL. Consistent with this, protein levels of SR-BI and ABCG1 were significantly lower in ob/ob hepatic and adipose tissue than in wildtype mice. Finally, labelled cholesterol concentrations were lower in ob/ob bile (67%, p<0.01) and faeces (76%, p<0.0001). Obesity causes impairment in RCT du...Continue Reading

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Citations

Jan 28, 2020·Nutrition Research Reviews·Mark Tomás Mc Auley
Mar 29, 2020·Frontiers in Physiology·Gaia FaveroRita Rezzani
Dec 5, 2019·Frontiers in Physiology·Xinguo JiangStanley G Rockson
Feb 26, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Katherine RiveraAttilio Rigotti

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Methods Mentioned

BETA
PCR

Software Mentioned

GeneSwitch system

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