Induction of oxidative DNA damage by ferric iron in mammalian cells

Carcinogenesis
A Hartwig, R Schlepegrell

Abstract

Ferric nitrilotriacetate (Fe-NTA) and ferric citrate (Fe-citrate) were compared with respect to their potential to induce oxidative DNA damage in V79 Chinese hamster cells. DNA base modifications, including 8-hydroxyguanine (7,8-dihydro-8-oxoguanine), were quantified by the frequency of lesions recognized by the bacterial Fpg protein (formamidopyrimidine-DNA glycosylase) in combination with the alkaline unwinding assay. Fe-NTA induced oxidative DNA damage in a time- and dose-dependent manner, yielding significant increases in Fpg-sensitive sites above background after incubation for 24 or 48 h with 500 and 250 microM respectively. At both time points the frequency of DNA base modifications exceeded the number of DNA strand breaks. In contrast, neither DNA strand breaks nor Fpg-sensitive sites were detected after treatment with Fe-citrate at concentrations up to 2 microM for 24 or 48 h; this inactivity of Fe-citrate was independent of the molar ratio of iron to ligand (1:1, 1:2, 1:10 or 1:20). The results indicate that the cellular damage induced by ferric iron depends strongly on the actual complex applied, possibly due to differences in the intracellular distribution, which in turn may affect the availability of iron for redox...Continue Reading

Citations

Jun 8, 2002·Toxicology Letters·Wojciech Bal, Kazimierz S Kasprzak
Jan 1, 1997·Free Radical Biology & Medicine·R Meneghini
Aug 6, 2002·Mutation Research·Michael GleiBeatrice L Pool-Zobel
Feb 8, 2008·BMC Gastroenterology·Donald J Messner, Kris V Kowdley
Mar 24, 2000·Archives of Biochemistry and Biophysics·U SattlerB Salles
Mar 28, 2007·Radiation and Environmental Biophysics·Fredric J BurnsRonghe Zhang
Jun 20, 2014·Biology·Franka J Rang, Johannes Boonstra
Dec 13, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Y KnöbelB L Pool-Zobel
Nov 28, 2012·Molecular Cell·Gregory HannumKang Zhang
Feb 26, 1999·Environmental and Molecular Mutagenesis·V C DunkelP Whittaker
May 20, 2005·Journal of Toxicology and Environmental Health. Part a·Xiaohong WuM Firoze Khan
Jan 28, 2017·Nutrition and Cancer·Donald J MessnerKris V Kowdley
May 28, 2014·International Journal of Toxicology·Monice M FiumeF Alan Andersen
Apr 13, 2021·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Habiba BouchabGabriele Saretzki

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