Induction of oxidative stress in rat epididymal sperm after exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin

Archives of Toxicology
C LatchoumycandaneP P Mathur

Abstract

The ability of 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD) to induce oxidative stress in various tissues of animals has been reported. The nature and mechanism of action of TCDD on the antioxidant system of sperm has not been studied. In the present study we have sought to investigate whether TCDD induces oxidative stress in the epididymal sperm of rats. Subchronic doses of TCDD (1, 10, and 100 ng/kg body weight per day) were administered orally to male Wistar strain rats for 45 days. After 24 h of the last treatment the rats were killed using diethyl ether. The epididymides were removed and cleared from the adhering tissues. Epididymal sperm were collected by cutting the epididymides into small pieces in Ham's F12 medium, and counted using a hemocytometer. The epididymal sperm counts in the TCDD-treated groups decreased in a dose-dependent manner from the control value of 8.2+/-0.14 x 10(8) to 5.31+/-0.15 x 10(8). Since a positive correlation (r=0.95; n=24) was observed between sperm count and DNA content of the epididymal sperm, DNA content was routinely used as an indicator of sperm count, and the results were expressed in terms of both protein and DNA. There was a significant decline in the activities of superoxide dismutas...Continue Reading

Citations

Sep 13, 2013·Ecotoxicology·Hiroko IshiniwaTsuneo Sekijima
Apr 17, 2002·Reproductive Toxicology·C LatchoumycandaneP P Mathur
Oct 12, 2013·Cell Stress & Chaperones·Kalai Selvi PalaniswamySaranya Ramalingam Singaravelu
Nov 13, 2012·Reproductive Toxicology·Emmanuel SommAriane Paoloni-Giacobino
Jul 4, 2012·Reproductive Toxicology·G LavranosR Angelopoulou
May 1, 2010·Environmental Toxicology and Pharmacology·Hiroko IshiniwaTsuneo Sekijima
Sep 2, 2010·Journal of Biochemical and Molecular Toxicology·S DhanabalanP P Mathur
Mar 29, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Seema JainPramod Kumari Mediratta
Jun 3, 2011·Toxicology and Industrial Health·Hai-Ping YinYing Wang
May 2, 2008·Molecular Reproduction and Development·Oli Sarkar, P P Mathur
Jun 20, 2020·Andrologia·Manesh Kumar Panner SelvamRalf Henkel
Jan 12, 2013·Toxicology and Industrial Health·Selcuk IlhanEngin Sahna
Aug 28, 2021·International Journal of Molecular Sciences·Laura GaspariSamir Hamamah

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