Induction of p53-independent apoptosis by simian virus 40 small t antigen

Journal of Virology
O GjoerupT M Roberts


Simian virus 40 small t antigen (st) is required for optimal transformation and replication properties of the virus. We find that in certain cell types, such as the human osteosarcoma cell line U2OS, st is capable of inducing apoptosis, as evidenced by a fragmented nuclear morphology and positive terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling staining of transfected cells. The cell death can be p53 independent, since it also occurs in p53-deficient H1299 cells. Genetic analysis indicates that two specific mutants affect apoptosis induction. One of these (C103S) has been frequently used as a PP2A binding mutant. The second mutant (TR4) lacks the final four amino acids of st, which have been reported to be unimportant for PP2A binding in vitro. However, TR4 unexpectedly fails to bind PP2A in vivo. Furthermore, a long-term colony assay reveals a potent colony inhibition upon st expression, and the behavior of st mutants in this assay reflects the relative frequency of nuclear fragmentation observed in transfections using the same mutants. Notably, either Bcl-2 coexpression or broad caspase inhibitor treatment could restore normal nuclear morphology. Finally, fluorescence-activated cell sorting analysis sugges...Continue Reading


Apr 1, 1991·Molecular and Cellular Biology·S I YangM C Mumby
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J A HoweS T Bayley
Jun 1, 1990·Journal of Virology·P JogK Rundell
Aug 1, 1988·Molecular and Cellular Biology·Y W ChoiS R Ross
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Sep 1, 1994·Molecular and Cellular Biology·J A FrostJ R Feramisco
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·S A McCarthyT Van Dyke
May 27, 1994·Biochimica Et Biophysica Acta·J J Manfredi, C Prives
Apr 1, 1993·Genes & Development·M Debbas, E White
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·G WatanabeR G Pestell
Aug 1, 1997·Molecular and Cellular Biology·V V OgryzkoB H Howard
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·A V Samuelson, S W Lowe
Apr 3, 1998·Journal of Virology·R Shtrichman, T Kleinberger
Apr 29, 1998·Annual Review of Physiology·K L King, J A Cidlowski
Aug 8, 1998·Genes & Development·E de StanchinaS W Lowe
Aug 26, 1998·FEBS Letters·T FukumoriA Adachi
Aug 28, 1998·Science·G Evan, T Littlewood
Nov 20, 1998·Molecular and Cellular Biology·K P MullaneB Schaffhausen
May 14, 1999·Trends in Biochemical Sciences·T A MillwardB A Hemmings
Aug 10, 1999·Experimental Cell Research·B WhalenJ M Lehman
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·R ShtrichmanT Kleinberger
Nov 5, 1999·Annual Review of Microbiology·A RoulstonP E Branton
Dec 28, 1999·Annual Review of Cell and Developmental Biology·I BudihardjoX Wang
Jan 5, 2000·Molecular Cell·A C PhillipsK H Vousden
Mar 14, 2000·Current Opinion in Cell Biology·D M Virshup
Apr 29, 2000·Nature·K M RyanK H Vousden
May 9, 2000·Journal of Virology·Q ShengB Schaffhausen
Sep 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·L ContiS Gessani
Sep 20, 2000·The Journal of Biological Chemistry·X WangN J Holbrook
Nov 1, 2000·Nature Cell Biology·P M OjalaT P Mäkelä
Mar 13, 2001·Seminars in Cancer Biology·K Rundell, R Parakati


Nov 17, 2007·Proceedings of the National Academy of Sciences of the United States of America·S AndrabiB Schaffhausen
May 28, 2014·Analytical Biochemistry·J F A ValenteFani Sousa
Jun 1, 2011·The Journal of Cell Biology·Kyung-Sun HeoJun-ichi Abe
Nov 2, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Soraia FerreiraCarla Cruz
Oct 16, 2015·Cell Death & Disease·C LaezzaM Bifulco
Jul 8, 2014·Oncogene·A T Pores FernandoT M Roberts
May 6, 2003·Biochimica Et Biophysica Acta·Christine Van Hoof, J Goris
Dec 18, 2016·Cell Stress & Chaperones·Stacey A MattisonAdrienne L Edkins
Apr 8, 2017·Biotechnology Letters·Xiaoling ZhouPingnan Sun
Jun 27, 2003·The Journal of Biological Chemistry·Fang BaiUsha P Andley
Aug 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Franceline JuillardJ Pedro Simas

Related Concepts

Simian Sarcoma Virus Proteins
Simian virus 40
Tumor Cells, Cultured
Papilloma, Shope
Virus Replication
Protein p53
Apoptosis, Intrinsic Pathway
B-Cell Leukemia 2 Family Proteins
Papillomavirus Infections

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