Induction of p53-independent growth inhibition in lung carcinoma cell A549 by gypenosides

Journal of Cellular and Molecular Medicine
Jung-Sen LiuBing-Huei Chen

Abstract

The objectives of this study are to investigate antiproliferative effect and mechanisms of bioactive compounds from Gynostemma pentaphyllum (G. pentaphyllum) on lung carcinoma cell A549. Saponins, carotenoids and chlorophylls were extracted and fractionated by column chromatography, and were subjected to high-performance liquid chromatography-mass spectrometry analyses. The saponin fraction, which consisted mainly of gypenoside (Gyp) XXII and XXIII, rather than the carotenoid and chlorophyll ones, was effective in inhibiting A549 cell growth in a concentration- and a time-dependent manner as evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The estimated half maximal inhibitory concentration (IC50 ) of Gyp on A549 cells was 30.6 μg/ml. Gyp was further demonstrated to induce an apparent arrest of the A549 cell cycle at both the S phase and the G2/M phase, accompanied by a concentration- and a time-dependent increase in the proportions of both the early and late apoptotic cells. Furthermore, Gyp down-regulated cellular expression of cyclin A and B as well as BCL-2, while up-regulated the expression of BAX, DNA degradation factor 35 KD, poly [ADP-ribose] polymerase 1, p53, p21 and caspase-3....Continue Reading

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Citations

Sep 8, 2017·Immunopharmacology and Immunotoxicology·Chunliu MiXuejun Jin
Feb 11, 2020·Natural Product Research·Wei-Ye CuiXiang-Lan Piao
Oct 1, 2017·Experimental and Therapeutic Medicine·Kung-Wen LuJing-Gung Chung
Mar 21, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jingxin MaKai Zheng
May 30, 2021·Bulletin of Experimental Biology and Medicine·N V EreminaA D Durnev

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Methods Mentioned

BETA
ELISA
protein assay
flow cytometry
glycosylation

Software Mentioned

SPSS

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