Induction of peritoneal sepsis increases the susceptibility of isolated hearts to a calcium paradox-mediated injury

Shock
Akira OmachiJames L Ferguson

Abstract

The present study was designed to test the hypothesis that induction of chronic peritoneal sepsis in rats would produce a more severe calcium paradox-mediated myocardial injury in isolated heart preparation than is seen in normal hearts, and that this would be inhibited by sucrose as in normal hearts. Male Sprague-Dawley rats were made septic using 200 mg of cecal material (obtained from a donor rat) suspended in 5 mL of 5% dextrose in sterile water D5 W/kg. In septic animals, the cecal material was injected in the peritoneum, while sham-septic animals received only D5 W/kg (5 mL/kg). A third group consisting of normal rats (no surgery) group was also included. Hearts were harvested from all three groups and were subjected to a calcium paradox-mediated injury in an isolated heart preparation. Hearts were perfused with Krebs-Henseleit (KH) medium and were allowed to stabilize, followed by a perfusion with Ca2+-free KH for 10 min. After this 10-min Ca2+-free KH perfusion, rats were reperfused with KH medium for 60 min. Ca2+-free KH medium was used in control experiments, while sucrose experiments were conducted with the same medium except that 150 mM sucrose replaced 75 mM NaCl. A marked decrease in ATP and phosphocreatine occurr...Continue Reading

References

Dec 1, 1976·Journal of Molecular and Cellular Cardiology·A B BionkA N Zimmerman
Jun 1, 1991·Journal of Molecular and Cellular Cardiology·R A AltschuldH M Piper
May 1, 1987·Pflügers Archiv : European journal of physiology·P Busselen
Nov 1, 1983·Journal of Molecular and Cellular Cardiology·W G NaylerM J Daly
May 1, 1997·Journal of Molecular and Cellular Cardiology·A C SharmaJ L Ferguson
Jun 10, 1998·Journal of Molecular and Cellular Cardiology·F M PowersW R Law

❮ Previous
Next ❯

Citations

Mar 1, 2005·Journal of Molecular and Cellular Cardiology·Akanksha GuptaAvadhesh C Sharma
May 29, 2007·Shock·Avadhesh C Sharma
Jun 7, 2014·Biochimica Et Biophysica Acta·Icksoo Lee, Maik Hüttemann
Jan 10, 2020·EMBO Molecular Medicine·Lise Van WyngeneClaude Libert

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.