Induction of peroxisomal changes in oligodendrocytes treated with 7-ketocholesterol: Attenuation by α-tocopherol

Biochimie
T NuryG Lizard

Abstract

The involvement of organelles in cell death is well established especially for endoplasmic reticulum, lysosomes and mitochondria. However, the role of the peroxisome is not well known, though peroxisomal dysfunction favors a rupture of redox equilibrium. To study the role of peroxisomes in cell death, 158 N murine oligodendrocytes were treated with 7-ketocholesterol (7 KC: 25-50 μM, 24 h). The highest concentration is known to induce oxiapoptophagy (OXIdative stress + APOPTOsis + autoPHAGY), whereas the lowest concentration does not induce cell death. In those conditions (with 7 KC: 50 μM) morphological, topographical and functional peroxisome alterations associated with modifications of the cytoplasmic distribution of mitochondria, with mitochondrial dysfunction (loss of transmembrane mitochondrial potential, decreased level of cardiolipins) and oxidative stress were observed: presence of peroxisomes with abnormal sizes and shapes similar to those observed in Zellweger fibroblasts, lower cellular level of ABCD3, used as a marker of peroxisomal mass, measured by flow cytometry, lower mRNA and protein levels (measured by RT-qPCR and western blotting) of ABCD1 and ABCD3 (two ATP-dependent peroxisomal transporters), and of ACOX1 a...Continue Reading

Citations

Sep 14, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Amira NamsiGérard Lizard
Jun 25, 2020·British Journal of Pharmacology·Thomas NuryGérard Lizard
Sep 5, 2020·Journal of Food Science·Ying LiuYuanfa Liu
Mar 27, 2020·Frontiers in Cellular Neuroscience·Ndidi-Ese UzorAndrey S Tsvetkov
Oct 22, 2020·Environmental Science and Pollution Research International·Sudhakaran SruthiGérard Lizard
Jul 22, 2019·The Journal of Steroid Biochemistry and Molecular Biology·Marco MalagutiSilvana Hrelia
Jan 14, 2020·Redox Biology·Amelia AndersonMatthew S O'Connor
Dec 16, 2020·Matrix Biology : Journal of the International Society for Matrix Biology·Laura ZeinSjoerd J L van Wijk
Jun 13, 2021·The Journal of Steroid Biochemistry and Molecular Biology·Imen GhzaielGérard Lizard

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