PMID: 6540173Jul 2, 1984Paper

Induction of phytoalexin synthesis in soybean. Stereospecific 3,9-dihydroxypterocarpan 6a-hydroxylase from elicitor-induced soybean cell cultures

European Journal of Biochemistry
M L HagmannH Grisebach

Abstract

A microsomal preparation from elicitor-challenged soybean cell suspension cultures catalyzes an NADPH-dependent and dioxygen-dependent 6a-hydroxylation of 3,9-dihydroxypterocarpan to 3,6a,9-trihydroxypterocarpan. The latter is a precursor for the soybean phytoalexin glyceollin. No reaction is observed with NADH. The 6a-hydroxylase is inhibited by cytochrome c. Optical rotatory dispersion spectra of the enzymatic product formed from racemic dihydroxypterocarpan and of the remaining unreacted substrate proved that the product has the natural (6aS, 11aS)-configuration and that hydroxylation proceeds with retention of configuration. The 6a-hydroxylase was also found in elicitor-challenged soybean seedlings. The results indicate that the 6a-hydroxylase is specifically involved in the biosynthesis of glyceollin.

References

Jan 1, 1976·Analytical Biochemistry·A Bensadoun, D Weinstein
Jul 15, 1974·FEBS Letters·H DiespergerH Sandermann
Aug 15, 1983·European Journal of Biochemistry·M L HagmannH Grisebach
Aug 1, 1982·Archives of Biochemistry and Biophysics·H Börner, H Grisebach

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Citations

Jan 1, 1997·Environmental Science and Pollution Research International·M SchalkD Werck-Reichhart
Jun 15, 2011·Organic Letters·Michael A Calter, Na Li
Jan 1, 1986·Plant Physiology·J A SweigardH D Vanetten
Mar 3, 1986·European Journal of Biochemistry·G Kochs, H Grisebach
Feb 15, 1989·European Journal of Biochemistry·E C Webb
Oct 3, 2009·The Journal of Pharmacology and Experimental Therapeutics·M Carla ZimmermannMatthew E Burow
Jan 1, 2009·Endocrinology·Stephen M BouéMatthew E Burow
Jan 1, 1995·Drug Metabolism and Drug Interactions·F Durst, D P O'Keefe
Jan 1, 1995·Drug Metabolism and Drug Interactions·D Werck-Reichhart
Jul 1, 1990·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·S CottrellD V Parke
Dec 1, 2009·Journal of Natural Products·Amarjit LuniwalPaul W Erhardt

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