Induction of pluripotent stem cells from mouse fibroblasts by four transcription factors

Cell Proliferation
Shinya Yamanaka

Abstract

Pluripotent stem cells, such as embryonic stem cells (ESCs), proliferate rapidly while maintaining pluripotency, namely, the ability to differentiate into various types of cells. Embryonic stem cells are promising donor sources for cell transplantation therapies. However, human ESCs are also associated with ethical issues regarding the use of human embryos and rejection reactions after allogenic transplantation. It may be possible to overcome these issues by generating pluripotent stem cells directly from a patient's somatic cells. That somatic cell nuclei acquire an embryonic stem-like status by fusion with ESCs suggests the existence of 'pluripotency-inducing' factors. Previous studies have recently shown that retrovirus-mediated transfection with four transcription factors (Oct-3/4, Sox2, KLF4 and c-Myc), which are highly expressed in ESCs, into mouse fibroblasts has resulted in generation of induced pluripotent stem (iPS) cells. iPS cells are similar to ESCs in morphology, proliferation, and pluripotency, judged by teratoma formation and chimaera contribution. If iPS cells can be derived from human somatic cells, then such cells may thus lead to important drug discoveries and advances in regenerative medicine.

References

Sep 1, 1976·Cell·R A Miller, F H Ruddle
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R Martin
Nov 6, 1998·Science·J A ThomsonJ M Jones
Apr 1, 2003·Molecular and Cellular Biology·Yoshimi TokuzawaShinya Yamanaka
May 30, 2003·Nature·Kazutoshi TakahashiShinya Yamanaka
Jul 6, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Shuyuan YaoJames Ellis
Aug 3, 2005·Nature Reviews. Molecular Cell Biology·Sovana Adhikary, Martin Eilers
Dec 24, 2005·Nature Reviews. Cancer·Benjamin D Rowland, Daniel S Peeper
Jan 10, 2006·Developmental Cell·Eran MeshorerTom Misteli
May 26, 2006·The EMBO Journal·Paul S KnoepflerRobert N Eisenman
Sep 7, 2006·Molecular and Cellular Biology·Yuhki NakatakeHitoshi Niwa
Jan 11, 2007·Cell Research·Guangjin Pan, James A Thomson
Jun 8, 2007·Nature·Keisuke OkitaShinya Yamanaka

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Citations

Jan 1, 2014·Advanced Drug Delivery Reviews·Maximilian Y EmmertSimon P Hoerstrup
Mar 4, 2014·Differentiation; Research in Biological Diversity·Dongshu Du, Xiangxin Lou
Aug 1, 2008·Chinese Journal of Traumatology = Zhonghua Chuang Shang Za Zhi·Cui-ping Zhang, Xiao-bing Fu
Aug 11, 2010·Antioxidants & Redox Signaling·Khawaja Husnain HaiderMuhammad Ashraf
Apr 23, 2008·Stem Cells and Development·Shi V Liu
Feb 9, 2011·Stem Cells and Development·Suresh RamakrishnaKwang-Hyun Baek
Mar 24, 2009·Cell Structure and Function·Fumiko NishiumiFumio Imamoto
Nov 10, 2010·The Review of Diabetic Studies : RDS·Isabelle Houbracken, Luc Bouwens
Mar 12, 2011·Regenerative Medicine·J Simon LunnEva L Feldman
Jul 1, 2011·Stem Cells International·Catriona KellyNeville H McClenaghan
Aug 19, 2015·Frontiers in Molecular Neuroscience·Arsalan AlizadehSoheila Karimi-Abdolrezaee
Oct 11, 2011·Expert Opinion on Therapeutic Targets·Sang Gyu ParkJong-Hyuk Sung
Jun 9, 2016·ACS Chemical Neuroscience·Nivanthika K WimalasenaRakesh Karmacharya
Jan 22, 2011·Progress in Neurobiology·Helena L A VieiraAlessandro Vercelli
Apr 7, 2010·Ageing Research Reviews·Meirong LiXiaobing Fu
Jul 10, 2016·Tissue Engineering. Part B, Reviews·Qingqing WuPing Wang
Sep 24, 2010·The Annals of Otology, Rhinology, and Laryngology·Naoki OchiKen-ichi Nibu
Jun 19, 2010·International Journal of Molecular Sciences·Chia-Wei HungCharn-Jung Chang
Mar 3, 2018·Cytotechnology·Pratheep SandrasaigaranRajesh Ramasamy
Nov 27, 2010·Cellular Reprogramming·Shiraz Gefen-HaleviSarah Ferber
Mar 26, 2014·American Journal of Medical Genetics. Part a·Peter J KochMaranke I Koster
May 28, 2010·Journal of Cellular Physiology·Vanessa M Y DingAndre B H Choo
Aug 4, 2010·International Journal of Colorectal Disease·Joanna PapailiouGeorge Theodoropoulos
May 19, 2012·Journal of Physics. Condensed Matter : an Institute of Physics Journal·M Di CarloP L San Biagio
Oct 1, 2011·Current Eye Research·Mikk PauklinDaniel Meller
Nov 17, 2019·International Journal of Molecular Sciences·Heng Liang Tan, Andre Choo
Nov 8, 2013·Canadian Journal of Zoology·T P YoshinoC J Bayne
Jul 8, 2008·Acta Biochimica Et Biophysica Sinica·Paul M Evans, Chunming Liu

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