Induction of prolactin expression and release in human preadipocytes by cAMP activating ligands

Biochemical and Biophysical Research Communications
Molly McFarland-ManciniNira Ben-Jonathan

Abstract

In addition to the pituitary, prolactin (PRL) in humans is produced at non-pituitary sites where it acts as a cytokine. We previously reported that PRL is expressed and released from breast adipose explants, raising the question as to the dynamics of its production and its regulation. Preadipocytes were isolated from breast adipose tissue obtained during breast reduction. PRL expression was transiently increased during early preadipocyte differentiation. Both isoproterenol, a beta-adrenergic receptor agonist, and PACAP, pituitary adenylate cyclase activating peptide, increased PRL expression, and release from preadipocytes. This stimulation was suppressed by several protein kinase inhibitors, suggesting involvement of multiple signaling pathways. Transfection of preadipocytes with a superdistal PRL promoter/luciferase reporter revealed two stimulatory domains and an inhibitory domain. These data establish the transcriptional regulation of adipocyte PRL by the superdistal PRL promoter, its transient expression during adipogenesis, and the stimulatory effect of catecholamines and PACAP.

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Citations

Apr 28, 2012·Journal of Molecular Neuroscience : MN·Katalin CsanakyAndrea Tamas
Jun 26, 2007·Diabetes, Obesity & Metabolism·T BrandebourgN Ben-Jonathan
Dec 7, 2007·Endocrine Reviews·Nira Ben-JonathanElizabeth W LaPensee
Jul 24, 2008·The Journal of Clinical Endocrinology and Metabolism·Eric R HugoNira Ben-Jonathan
Mar 13, 2013·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Juan M VélezEsther Ocharán
May 13, 2011·Trends in Endocrinology and Metabolism : TEM·Allison J Richard, Jacqueline M Stephens
May 9, 2009·British Journal of Pharmacology·Martin T GerickeKatharina Spanel-Borowski
Sep 14, 2010·International Journal of Urology : Official Journal of the Japanese Urological Association·Chong Won BakSuye Sung
Sep 24, 2013·JAK-STAT·Peng Zhao, Jacqueline M Stephens
Apr 4, 2014·Molecular Endocrinology·Robert J Marano, Nira Ben-Jonathan
Feb 26, 2010·The American Journal of Clinical Nutrition·Gertruud Cm BakkerHenk Fj Hendriks

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