Induction of protein catabolism and the ubiquitin-proteasome pathway by mild oxidative stress

Cancer Letters
Maria Cristina C Gomes-Marcondes, M J Tisdale

Abstract

Muscle wasting in cancer cachexia is associated with increased levels of malondialdehyde (MDA) in gastrocnemius muscles, suggesting an increased oxidative stress. To determine whether oxidative stress contributes to muscle protein catabolism, an in vitro model system, consisting of C2C12 myotubes, was treated with either 0.2 mM FeSO4, 0.1 mM H2O2, or both, to replicate the rise in MDA content in cachexia. All treatments caused an increased protein catabolism and a decreased myosin expression. There was an increase in the proteasome chymotrypsin-like enzyme activity, while immunoblotting showed an increased expression of the 20S proteasome alpha-subunits, p42, and the ubiquitin-conjugating enzyme, E214k. These results show that mild oxidative stress increases protein degradation in skeletal muscle by causing an increased expression of the major components of the ubiquitin-proteasome pathway.

Citations

Mar 18, 2006·Apoptosis : an International Journal on Programmed Cell Death·H ShenQ Y Liu
Sep 25, 2010·Current Hypertension Reports·Marina Bayeva, Hossein Ardehali
Aug 21, 2010·Journal of Physiology and Biochemistry·Miroslav KovarikMilan Holecek
Feb 9, 2012·Journal of Physiology and Biochemistry·Miroslav KovarikMilan Holecek
May 14, 2005·Mutation Research·Heidrun Ellinger-ZiegelbauerHans Juergen Ahr
Apr 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kerri E RiegerGilbert Chu
Aug 19, 2009·Antioxidants & Redox Signaling·Esther Barreiro, Sabah N A Hussain
Sep 10, 2009·Antioxidants & Redox Signaling·Judith Marin-CorralEsther Barreiro
Aug 28, 2004·Molecular Biology of the Cell·Roberta KiffinAna Maria Cuervo
Jul 25, 2007·Bioinformatics·Yanxin ShiZiv Bar-Joseph
Sep 16, 2011·The Journal of Infectious Diseases·Nicole J LeCapitainePatricia E Molina
Oct 6, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jennifer MurrayJanice M Huss
Jun 14, 2003·Current Opinion in Clinical Nutrition and Metabolic Care·Paola Costelli, Francesco M Baccino
Jun 15, 2007·Current Opinion in Clinical Nutrition and Metabolic Care·Alessandro LavianoFilippo Rossi Fanelli
Jan 26, 2011·Development, Growth & Differentiation·Antonio Hernandes Chaves NetoCarmen Veríssima Ferreira
Sep 14, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Andreas N KavazisScott K Powers
Aug 19, 2006·American Journal of Respiratory and Critical Care Medicine·Coen A C OttenheijmP N Richard Dekhuijzen
Apr 7, 2007·American Journal of Respiratory and Critical Care Medicine·Coen A C OttenheijmP N Richard Dekhuijzen
Nov 17, 2004·The Journal of Clinical Investigation·R Bridge Hunter, Susan C Kandarian
Jan 26, 2008·Respiratory Research·Coen A C OttenheijmRichard P N Dekhuijzen
Nov 27, 2004·Bioscience, Biotechnology, and Biochemistry·Kazuki NakashimaHiroyuki Abe
Jan 5, 2014·Reproductive Biology and Endocrinology : RB&E·Bread Cruz, Maria C C Gomes-Marcondes
Jun 6, 2014·BioMed Research International·Sajal GuptaAshok Agarwal
Dec 14, 2011·Revue des maladies respiratoires·M-A CaronF Maltais
Jul 6, 2014·Pathophysiology : the Official Journal of the International Society for Pathophysiology·F H BorgesR Cecchini
Dec 18, 2015·Journal of Cachexia, Sarcopenia and Muscle·Cathleen DrescherJochen Springer
Feb 6, 2016·BMC Cancer·Bread Leandro Gomes CruzMaria Cristina Cintra Gomes-Marcondes
Dec 29, 2015·Free Radical Biology & Medicine·Mohamad AssiAmélie Rébillard
Dec 23, 2008·FEBS Letters·Esther BarreiroFrancisco J López-Soriano
Oct 24, 2013·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Jair TononRubens Cecchini
Feb 24, 2016·Oxidative Medicine and Cellular Longevity·Aline V BacurauPatricia C Brum
Aug 7, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Antonio Hernandes Chaves NetoCarmen Veríssima Ferreira

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