Induction of pseudo-periodic oscillation in voltage-gated sodium channel properties is dependent on the duration of prolonged depolarization

The European Journal of Neuroscience
Sriparna MajumdarS K Sikdar

Abstract

The neuronal voltage-gated sodium channels play a vital role in the action potential waveform shaping and propagation. Here, we report the effects of prolonged depolarization (1-160 s) on the detailed kinetics of activation, fast inactivation and recovery from slow inactivation in the rNa(v)1.2a voltage-gated sodium channel alpha-subunit expressed in Chinese hamster ovary (CHO) cells. Wavelet analysis revealed that the duration and amplitude of a prolonged sustained depolarization altered all the steady state and kinetic parameters of the channel in a pseudo-oscillatory fashion with time-variable period and amplitude, often superimposed on a linear trend. The half steady state activation potential showed a reversible depolarizing shift of 5-10 mV with duration of prolonged depolarization, while half steady state inactivation potential showed a hyperpolarizing shift of 43-55 mV. The time periods for most of the parameters relating to activation and fast and slow inactivation, lie close to 28-30 s, suggesting coupling of these kinetic processes through an oscillatory mechanism. Co-expression of the beta1-subunit affected the time periods of oscillation (close to 22 s for alpha + beta1) in steady state activation parameters. Appli...Continue Reading

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Citations

Apr 6, 2006·The Journal of Membrane Biology·S Majumdar, S K Sikdar
Sep 4, 2007·The Journal of Membrane Biology·Tapan K Nayak, S K Sikdar
Jul 13, 2006·Biochemical and Biophysical Research Communications·Sriparna Majumdar, Sujit K Sikdar
Jun 15, 2007·Biochemical and Biophysical Research Communications·Sriparna Majumdar, Sujit K Sikdar

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