Induction of RNA interference in Caenorhabditis elegans by RNAs derived from plants exhibiting post-transcriptional gene silencing

Nucleic Acids Research
Alexandra BoutlaMartin Tabler

Abstract

The term 'gene silencing' refers to transcriptional and post-transcriptional control of gene expression. Related processes are found across kingdoms in plants and animals. We intended to test whether particular RNA constituents of a silenced plant can induce silencing in an animal. We generated Nicotiana benthamiana lines that expressed green fluorescent protein (GFP) from a transgene. Plants in which GFP expression was spontaneously silenced showed siRNAs characteristic of post-transcriptional gene silencing (PTGS). RNA extracts prepared from silenced plants were injected into a GFP-expressing strain of Caenorhabditis elegans, where they induced RNA interference (RNAi). Extracts from non-silenced plants were inactive. This directly demonstrates a relationship and a mechanistic link between PTGS and RNAi. Controls confirmed that the silencing agent was an RNA. Size fractionation on denaturing gels revealed that an RNA of approximately 85 nt was most active in inducing silencing in the worm. Northern blot analysis of the region in question did not detect a prominent GFP-specific RNA of sense or antisense polarity, indicating that the RNA species which induced silencing was present only in low concentration or did not hybridize d...Continue Reading

References

Feb 11, 1994·Science·M ChalfieD C Prasher
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·J HaseloffS Hodge
Apr 1, 1997·Antisense & Nucleic Acid Drug Development·J Moosbauer, M Tabler
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·C Cogoni, G Macino
Oct 23, 1997·Nature·O Voinnet, D C Baulcombe
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·H NgôE Ullu
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·M K MontgomeryA Fire
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·L Misquitta, B M Paterson
Jun 17, 1999·FEMS Microbiology Reviews·A W Nicholson
Sep 2, 1999·Current Biology : CB·D C Baulcombe
Sep 16, 1999·Biochemical and Biophysical Research Communications·A WargeliusA Fjose
Jan 8, 2000·Developmental Biology·Y X LiM L Kirby
Feb 3, 2000·Nature Cell Biology·F Wianny, M Zernicka-Goetz
Jun 13, 2000·The Journal of Biological Chemistry·N A TchurikovY B Golova
Oct 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·M FagardH Vaucheret
Feb 7, 2001·Genes & Development·S M ElbashirT Tuschl
Mar 7, 2001·Current Biology : CB·E J FinneganP Waterhouse
Mar 10, 2001·Genes & Development·P A Sharp
Mar 16, 2001·Current Opinion in Genetics & Development·M A MatzkeV B Vance
Jun 26, 2001·Science·V Vance, H Vaucheret
Jul 4, 2001·Trends in Plant Science·P M WaterhouseE J Finnegan
Jul 19, 2001·Nature·P M WaterhouseT Lough

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Citations

Oct 26, 2005·Plant Molecular Biology·Edyta KościańskaMartin Tabler
Jun 29, 2010·Transgenic Research·Athanasios DalakourasKriton Kalantidis
Nov 27, 2007·Plant Methods·Bess L Chau, Kevin Aw Lee
Mar 1, 2005·Molecular Plant Pathology·Stephen C WhissonJohn T Jones
Nov 18, 2003·The Plant Journal : for Cell and Molecular Biology·Shuai ChenFrederik Börnke
May 20, 2003·The Plant Journal : for Cell and Molecular Biology·Christophe LacommeIngo Hein
Sep 4, 2007·Plant Biotechnology Journal·Tracy A ValentineKarl J Oparka
Jun 15, 2006·The Plant Journal : for Cell and Molecular Biology·Barthélémy TournierKriton Kalantidis
Jul 21, 2012·Plant Biology·A A UdristeN Cucu
May 18, 2004·Gene·Bartel VanholmeGodelieve Gheysen

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