Induction of secondary metabolism in grape cell cultures by jasmonates

Functional Plant Biology : FPB
Claudio D' OnofrioPaul K Boss

Abstract

The use of a genetic approach to study the biosynthetic pathways leading to the production of secondary metabolites in grapes is difficult given the long generation times and difficulty in transforming this species. In the present study, GC/MS and microarray experiments were used to identify compounds produced in grape cell cultures in response to jasmonates and to examine the expression of genes from pathways that produce these secondary metabolites. Methyl jasmonate (MeJA) and jasmonic acid (JA) treatments resulted in the production of at least 25 compounds with sesquiterpene-like mass spectra in the cell cultures. A significantly greater amount of proanthocyanidins was produced in the MeJA-treated cell cultures compared with controls and stilbene biosynthesis was induced in both MeJA- and JA-treated cells. Salicylic acid (SA) suppressed the MeJA-associated increase in sesquiterpenes and proanthocyanidins, but SA did not suppress the stilbene production induced by MeJA treatment. The mechanism by which jasmonates induced secondary metabolite production in cultured grape cells varied depending on the pathway. The increased production of proanthocyanidins and stilbenes was associated with the induction of all of the genes in as...Continue Reading

References

Jan 13, 2001·Current Opinion in Immunology·B P ThommaB P Cammue
Mar 30, 2001·Nature·C M De MoraesJ H Tumlinson
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·J GörlachJ Schmid
May 2, 2002·Journal of Agricultural and Food Chemistry·Philippe JeandetMarielle Adrian
Jun 13, 2002·The Plant Journal : for Cell and Molecular Biology·Gen-ichiro ArimuraJunji Takabayashi
Aug 16, 2002·Current Opinion in Plant Biology·Barbara N Kunkel, David M Brooks
Aug 21, 2003·Journal of Agricultural and Food Chemistry·Roger PezetOlivier Viret
Sep 13, 2003·Journal of Applied Microbiology·K A HammerT V Riley
Jan 1, 2004·Plant Physiology·Keyan Zhu-SalzmanHisashi Koiwa
Jan 30, 2004·Proceedings of the National Academy of Sciences of the United States of America·Juergen EngelberthJames H Tumlinson
Aug 25, 2004·Plant Physiology·Natalia DudarevaJonathan Gershenzon
Oct 7, 2004·Phytochemistry·Elio G W M SchijlenArnaud G Bovy
Feb 17, 2005·Pest Management Science·Matías GarcíaMarta E Sosa
Feb 22, 2005·The New Phytologist·Richard A DixonShashi B Sharma
May 28, 2005·The Plant Journal : for Cell and Molecular Biology·Dorothea ThollEran Pichersky
Jul 26, 2005·Current Opinion in Plant Biology·Oscar Lorenzo, Roberto Solano
Sep 24, 2005·Science·Iris F KappersHarro J Bouwmeester
Jan 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Christiane SchneeJörg Degenhardt
Jan 26, 2006·Plant Biology·G J M Beckers, S H Spoel
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth
May 30, 2006·Nature Reviews. Drug Discovery·Joseph A Baur, David A Sinclair
Nov 8, 2006·Nucleic Acids Research·Kevin L ChildsAgnes P Chan
Nov 23, 2006·Journal of Agricultural and Food Chemistry·Assia BelhadjJean-Michel Mérillon
Aug 28, 2007·Nature·Olivier JaillonUNKNOWN French-Italian Public Consortium for Grapevine Genome Characterization
Jan 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Laurens PauwelsAlain Goossens
May 9, 2008·Journal of Agricultural and Food Chemistry·Claudia WoodMarkus J Herderich
Nov 28, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Juri BattilanaM Stella Grando
Sep 1, 2002·Molecular Plant Pathology·Richard A DixonLiangjiang Wang

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Citations

Apr 16, 2015·Journal of Experimental Botany·Dong DuanPeter Nick
Nov 10, 2010·Applied Biochemistry and Biotechnology·Inga MewisChristian Ulrichs
Dec 17, 2014·Journal of Integrative Plant Biology·Christine BöttcherChristopher Davies
Dec 11, 2019·Journal of the Science of Food and Agriculture·Gicele S De BonaSimone Vincenzi
Jun 3, 2021·Plant Physiology and Biochemistry : PPB·Viviana MartinsArnaud Lanoue

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