Induction of secretory pathway components in yeast is associated with increased stability of their mRNA

The Journal of Cell Biology
Maureen HydeDavid I Meyer

Abstract

The overexpression of certain membrane proteins is accompanied by a striking proliferation of intracellular membranes. One of the best characterized inducers of membrane proliferation is the 180-kD mammalian ribosome receptor (p180), whose expression in yeast results in increases in levels of mRNAs encoding proteins that function in the secretory pathway, and an elevation in the cell's ability to secrete proteins. In this study we demonstrate that neither the unfolded protein response nor increased transcription accounts for membrane proliferation or the observed increase in secretory pathway mRNAs. Rather, p180-induced up-regulation of certain secretory pathway transcripts is due to a p180-mediated increase in the longevity of these mRNA species, as determined by measurements of transcriptional activity and specific mRNA turnover. Moreover, we show that the longevity of mRNA in general is substantially promoted through the process of its targeting to the membrane of the endoplasmic reticulum. With respect to the terminal differentiation of secretory tissues, results from this model system provide insights into how the expression of a single protein, p180, could result in substantial morphological and functional changes.

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Citations

Nov 28, 2008·Molecular Biology of the Cell·Payam BenyaminiDavid I Meyer
Feb 13, 2003·Nucleic Acids Research·Antonello RomaniSaverio Alberti
Jan 5, 2006·Applied and Environmental Microbiology·Anoushka DavéDavid B Archer
Jun 15, 2005·Annual Review of Biochemistry·Martin Schröder, Randal J Kaufman
Nov 20, 2002·The Journal of Clinical Investigation·Randal J Kaufman
Jan 7, 2014·PLoS Computational Biology·Shelly Mahlab, Michal Linial
Mar 4, 2014·PLoS Pathogens·Jessica L MartinNicola S Carter
Jun 9, 2004·Biochemical and Biophysical Research Communications·Russell J DiefenbachAnthony L Cunningham
Nov 18, 2010·Molecular Systems Biology·Changhui MaoHinrich Boeger
Dec 18, 2004·Mutation Research·Martin Schröder, Randal J Kaufman
Jan 16, 2003·Yeast
Dec 27, 2011·The Journal of Biological Chemistry·David W Reid, Christopher V Nicchitta
Jul 10, 2019·Bioscience Reports·Jing MaFanling Meng
Jul 18, 2018·International Journal of Molecular Medicine·Dongbin ParkYoonsoo Hahn
Aug 7, 2019·Nature Microbiology·Yaw Shin OoiJan E Carette

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Methods Mentioned

BETA
glycosylation
electron microscopy
protein folding
PCR
protein assay

Software Mentioned

ImageQuant

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