Induction of senescence in primary glioblastoma cells by serum and TGFβ

Scientific Reports
Ritesh KumarIan A J Lorimer

Abstract

Glioblastoma is the most common type of adult brain tumour and has a median survival after diagnosis of a little more than a year. Glioblastomas have a high frequency of mutations in the TERT promoter and CDKN2A locus that are expected to render them resistant to both replicative and oncogene-induced senescence. However, exposure of PriGO8A primary glioblastoma cells to media with 10% serum induced a senescence-like phenotype characterized by increased senescence-associated β galactosidase activity, PML bodies and p21 and morphological changes typical of senescence. Microarray expression analysis showed that 24 h serum exposure increased the expression of genes associated with the TGFβ pathway. Treatment of PriGO8A cells with TGFβ was sufficient to induce senescence in these cells. The response of PriGO8A cells to serum was dependent on basal expression of the TGFβ activator protein thrombospondin. Primary glioblastoma cells from three additional patients showed a variable ability to undergo senescence in response to serum. However all were able to undergo senescence in response to TGFβ, although for cells from one patient this required concomitant inhibition of Ras pathway signalling. Primary glioblastoma cells therefore retai...Continue Reading

References

Apr 10, 1999·Genes & Development·M KretzschmarJ Massagué
Dec 1, 1961·Experimental Cell Research·L HAYFLICK, P S MOORHEAD
Nov 11, 2003·Nature·Fabrizio d'Adda di FagagnaStephen P Jackson
Dec 19, 2003·Nucleic Acids Research·Minoru KanehisaMasahiro Hattori
Aug 5, 2005·Nature·Chrysiis MichaloglouDaniel S Peeper
Oct 10, 2006·Biochimica Et Biophysica Acta·Clemens A Schmitt
Dec 1, 2006·Nature·Raffaella Di MiccoFabrizio d'Adda di Fagagna
Nov 15, 2007·Nature Reviews. Molecular Cell Biology·Bernhard Schmierer, Caroline S Hill
Feb 5, 2008·Genes & Development·Luis I ToledoOscar Fernandez-Capetillo
Jul 25, 2008·PLoS Biology·Alexander R PicoChris Evelo
Oct 25, 2008·Cell Cycle·Zoya N Demidenko, Mikhail V Blagosklonny
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Feb 19, 2009·Molecular and Cellular Biology·David C ClarkeXuedong Liu
May 15, 2009·Nature Reviews. Cancer·Tarek Abbas, Anindya Dutta
Jan 19, 2010·Annual Review of Pathology·Jean-Philippe CoppéJudith Campisi
Sep 15, 2010·Journal of Immunological Methods·Takatoku Oida, Howard L Weiner
Nov 9, 2010·Progress in Neurobiology·Victoria A Rafalski, Anne Brunet
Nov 29, 2011·Oncogene·J A PagetI A J Lorimer
Mar 1, 2012·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Ryan R Gordon, Peter S Nelson
Apr 20, 2012·PloS One·Brad J ZerlankoDavid Wotton
Mar 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Patrick J KillelaHai Yan
Apr 17, 2013·BMC Bioinformatics·Edward Y ChenAvi Ma'ayan
Oct 15, 2013·Cell·Cameron W BrennanUNKNOWN TCGA Research Network
Oct 18, 2013·Nature Reviews. Cancer·Michael PickupHarold L Moses
Jan 23, 2014·Genes & Development·Rafik SalamaMasashi Narita

❮ Previous
Next ❯

Citations

Mar 19, 2020·Biomolecules·Mahmut MijitAntonio Giordano
Sep 24, 2019·Current Aging Science·Joan Smith-Sonneborn
Apr 5, 2018·Acta Neuropathologica·Floris P BarthelRoel G W Verhaak
Mar 23, 2018·International Journal of Molecular Sciences·Elzbieta PawlowskaJanusz Blasiak

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
X-Ray

Software Mentioned

cBioportal
ChEA
Affymetrix
Wikipathways
Enrichr
Biocarta
DAVID Bioinformatics Resources

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.